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Rifampicin (CAS 13292-46-1)

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Alternate Names:
Rifampicin is also known as Rifampin.
Application:
Rifampicin is a rifamycin antibiotic that potently inhibits RNA polymerase, blocks RNA synthesis, and is an activator of PXR.
CAS Number:
13292-46-1
Purity:
≥95%
Molecular Weight:
823.00
Molecular Formula:
C43H58N4O12
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Rifampicin is a well-studied compound that is extensively used in research for its action as a potent inhibitor of bacterial RNA polymerase. The compound′s specificity for the bacterial enzyme makes it a key tool in molecular biology for investigating the transcriptional processes of bacteria. In the study of gene expression and regulation, rifampicin is utilized to understand the initiation and elongation phases of RNA synthesis, as well as the effect of transcriptional inhibition on bacterial cell viability. Research involving rifampicin also includes examining the development of antibiotic resistance, where the compound′s interaction with the RNA polymerase subunit provides insights into the mechanisms by which bacteria adapt to evade antibacterial agents. Additionally, rifampicin is of interest in the field of synthetic biology, where it is employed in the design of controlled gene expression systems due to its ability to selectively inhibit transcription.


Rifampicin (CAS 13292-46-1) References

  1. Simultaneous determination of rifampicin and sulbactam in mouse plasma by high-performance liquid chromatography.  |  Aparicio, I., et al. 2006. Biomed Chromatogr. 20: 748-52. PMID: 16252264
  2. Rifampicin attenuates the MPTP-induced neurotoxicity in mouse brain.  |  Oida, Y., et al. 2006. Brain Res. 1082: 196-204. PMID: 16515773
  3. Chromosomal composition of micronuclei in mouse bone marrow treated with rifampicin and nicotine, analyzed by multicolor fluorescence in situ hybridization with pancentromeric DNA probe.  |  Attia, SM. 2007. Toxicology. 235: 112-8. PMID: 17420080
  4. Effect of L-cycloserine on cellular responses mediated by macrophages and T cells.  |  Cho, JY. 2007. Biol Pharm Bull. 30: 2105-12. PMID: 17978484
  5. Simultaneous determination of rifampicin and levofloxacin concentrations in catheter segments from a mouse model of a device-related infection by liquid chromatography/electrospray ionization tandem mass spectrometry.  |  Bao, D., et al. 2008. J Pharm Biomed Anal. 46: 723-7. PMID: 18178051
  6. Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection.  |  Fernández-Torres, R., et al. 2008. Anal Chim Acta. 608: 204-10. PMID: 18215652
  7. Rapid kidney changes resulting from glycosphingolipid depletion by treatment with a glucosyltransferase inhibitor.  |  Shukla, GS., et al. 1991. Biochim Biophys Acta. 1083: 101-8. PMID: 1827738
  8. Rifampicin reduces alpha-synuclein in a transgenic mouse model of multiple system atrophy.  |  Ubhi, K., et al. 2008. Neuroreport. 19: 1271-6. PMID: 18695506
  9. Rifampicin attenuates experimental autoimmune encephalomyelitis by inhibiting pathogenic Th17 cells responses.  |  Ma, K., et al. 2016. J Neurochem. 139: 1151-1162. PMID: 27774592
  10. Rifampicin-Induced Hepatic Lipid Accumulation: Association with Up-Regulation of Peroxisome Proliferator-Activated Receptor γ in Mouse Liver.  |  Huang, JH., et al. 2016. PLoS One. 11: e0165787. PMID: 27806127
  11. Rifampicin ameliorates lipopolysaccharide-induced cognitive and motor impairments via inhibition of the TLR4/MyD88/NF-κB signaling pathway in mice.  |  Bi, W., et al. 2021. Neurol Res. 43: 358-371. PMID: 33749522
  12. Drug Resistance of Mouse Somatic Cells to Rifampicin in Experimental Tuberculosis.  |  Nikonenko, BV., et al. 2021. Bull Exp Biol Med. 171: 53-55. PMID: 34050415
  13. Use of colistin with rifampicin, trimethoprim-sulfamethoxazole and teicoplanin in Acinetobacter mouse infection model.  |  Yesil, C., et al. 2022. Future Microbiol. 17: 665-671. PMID: 35400195
  14. Inhibition of sphingolipid synthesis by cycloserine in vitro and in vivo.  |  Sundaram, KS. and Lev, M. 1984. J Neurochem. 42: 577-81. PMID: 6693888
  15. L-cycloserine, an inhibitor of sphingolipid biosynthesis, inhibits HIV-1 cytopathic effects, replication, and infectivity.  |  Mizrachi, Y., et al. 1996. J Acquir Immune Defic Syndr Hum Retrovirol. 11: 137-41. PMID: 8556395

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rifampicin, 1 g

sc-200910
1 g
$95.00

Rifampicin, 5 g

sc-200910A
5 g
$322.00

Rifampicin, 100 g

sc-200910B
100 g
$663.00

Rifampicin, 250 g

sc-200910C
250 g
$1438.00

Why the rifampin is slightly soluble in water?

Asked by: Sivaram
Thank you for your question. The solubility information we have available for this chemical in water is as follows: Soluble in water (pH 4.3) (1.3 mg/ml), and water (pH 7.3) (2.5 mg/ml) at 25° C.
Answered by: Technical Support
Date published: 2024-09-25

I prepared 150 mg/ml stock solution of Rifampin (Rifampicin from MP biomedicals) by using methanol, in this solution it is not 100% dissolved for 150mg/ml. kindly suggest how to prepare 150mg/ml stock and by using which solvent?

Asked by: moni1901
Thank you for your question. Rifampicin is soluble in water (pH 4.3) (1.3 mg/ml), methanol (25 mg/ml), DMSO (25 mg/ml), dimethyl sulfoxide, tetrahydrofuran, and water (pH 7.3) (2.5 mg/ml at 25 °C).
Answered by: Technical Support
Date published: 2021-09-26
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Rated 5 out of 5 by from EchchgaddaEchchgadda, et. al. (PubMed ID 17595319) treated primary mouse primary hepatocytes with rifampicin in order to induce PXR recruitment. They found it peaked at 2.5 hours past treatment. -SCBT Publication Review
Date published: 2015-04-07
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Rifampicin is rated 5.0 out of 5 by 1.
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