1-800-457-3801
β-Actin (C4): sc-47778. Western blot analysis of β-Actin expression in HeLa (A), Jurkat (B), K-562 (C) and A-431 (D) whole cell lysates. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
goat anti-mouse IgG-HRP: sc-2005
4.8(39)
Write a reviewAsk a question- supplied at 200 µg in 0.5 ml volume
- horseradish peroxidase conjugated secondary antibody
- recommended for use in Western blotting at a dilution of 1:500 - 1:10000. Starting dilution: 1:2000
- goat anti-mouse IgG-HRP has been discontinued and replaced with our new, and much superior, Isotype Specific panel of recombinant Mouse IgG Binding Proteins.
SEE ALSO...
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
Ordering Information
goat anti-mouse IgG-HRP has been discontinued and replaced with our new, and much superior, Isotype Specific panel of recombinant Mouse IgG Binding Proteins.
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
m-IgG Fc BP-HRP | sc-525409 | 50 µg/0.5 ml | $92.00 | |||
m-IgGκ BP-HRP | sc-516102 | 200 µg/0.5 ml | $92.00 | |||
m-IgG1 BP-HRP | sc-525408 | 100 µg/0.5 ml | $92.00 | |||
m-IgGλ BP-HRP | sc-516132 | 200 µg/0.5 ml | $92.00 |
Does this antibody recognize and bind to mouse IgG2a, κ?
Asked by: Anonymous
Thank you for your question. I am very sorry that there is no data available for chemicals. Thank you.
Answered by: Jiajun Z
Date published: 2025-08-07
Can sc-525409 IgG-Fc be used for ELISA?
Asked by: Derek
Thank you for your question. sc-525409 m-IgG Fc BP-HRP is recommended for western blot and IHC.
Answered by: BlakeJ
Date published: 2024-12-19
If I want to use the 200 ug/ml antibody at a 1:5000 dilution, what is the dilution procedure?
Asked by: Recyclops
Thank you for your question. For that dilution you would have 1 µl antibody in 5 ml in a dilution buffer of your choice, such as 5 % UltraCruz blocking reagent in TBS-T.
Answered by: Tech Support Europe
Date published: 2022-09-05
Can this antidody be used to detect IgM kappa light chain for WB?
Asked by: Wayneman
Thank you for your question. This goat anti-mouse IgG-HRP: sc-2005 is now discontinued. It is recommended for detection of mouse IgG. We do not know if it may also detect mouse IgM. We would recommend using our m-IgGκ BP-HRP: sc-516102 which has been validated for the detection of mouse IgM kappa light chain for WB.
Answered by: Technical Support
Date published: 2024-12-28
Excuse me, can i ask for the specifities of goat anti-mouse IgG-HRP? I've used It in laboratory for a reserch an i need know the isotype, origin, way of use,...
Asked by: Claudio96
Thank you for your question. This discontinued goat anti-mouse secondary antibody is no longer available. It is a secondary antibody made in goat, it consists of a pool of IgG, so not a specific isotype. Please reach out to Technical Service or your local distributor if you have any further questions, or would like help choosing an available secondary reagent.
Answered by: Tech Service
Date published: 2019-10-14
Is this antibody (goat anti-mouse IgG-HRP: sc-2005) able to detect both light and heavy chain in Western Blot?
Asked by: FlottefzmbLotte1234
Thank you for your question. Goat anti-mouse IgG-HRP: sc-2005 has been raised against mouse IgG and recognizes both the light and the heavy chain of mouse IgG antibodies. This polyclonal antibody is no longer available. Instead we offer m-IgGκ BP-HRP: sc-516102 and other secondary binding proteins which you can find by following this link:
https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: SCBT Heidelberg Support
Date published: 2020-01-16
HOW MUCH THIS ANTIBODY ?
Asked by: Saif
We have discontinued all our polyclonal antibodies, unfortunately, this antibody doesn't exist anymore. If you want a quotation for any of our alternative antibodies, please send us an email to uk@scbt.com with your complete delivery address and we will send you a detailed quotation
Answered by: Technical Support Europe
Date published: 2018-07-17
One of our users asked this following question about the source of antibody: Scientific name of the goat from which your products are derived (Capra hircus?). Please let me know ASAP so I can tell him. Thank you!
Asked by: Frankh
Thank you for your question. All our goat polyclonal antibodies, including this secondary antibody, have been discontinued. However, I can confirm that they are from domestic goats of the species Capra hircus. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-02-17
- y_2025, m_9, d_24, h_6CST
- bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
- cp_1, bvpage1
- co_hasquestionsanswers, tq_8
- loc_en_US, sid_2005, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
- clientName_scbt
- bvseo_sdk, java_sdk, bvseo-4.0.0
- CLOUD, getContent, 116ms
- QUESTIONS, PRODUCT
Rated 5 out of
5 by
SaikatB from
ExcellentVery clear detection of protein bands with minimum background
Date published: 2023-02-03
Rated 5 out of
5 by
W8608 from
Excellent antibody for ELISAWe used this secondary antibody for ELISA to detect pathogen-specific antibodies generated in mice and it worked beautifully.
Date published: 2022-12-25
Rated 5 out of
5 by
KaWi from
Good secondary antibodyThe antibody worked well with the primary antibodies we used (sc-13515 and sc-398788)
Date published: 2021-07-30
Rated 5 out of
5 by
Chrysa from
Excellent antibodyWorked really good at a dilution of 1:5000 in Western blot
Date published: 2021-05-24
Rated 5 out of
5 by
Anonymous from
excellent antibodyexcellent antibody. we've been using this for years and it is very good.
Date published: 2020-10-31
Rated 5 out of
5 by
Tan4 from
Good ProductAntibody works well with western blotting analysis with cancer cell line lysate.
Date published: 2019-12-26
Rated 5 out of
5 by
Kinsella Lab from
Secondary AntibodyExcellent secondary antibody for western blotting.
Date published: 2019-05-30
Rated 4 out of
5 by
Claire_1 from
Works goodThis secondary antibody works quite good for immunoblot at a concentration of 1:10,000.
Date published: 2018-07-30
- y_2025, m_9, d_24, h_6
- bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
- cp_1, bvpage1
- co_hasreviews, tv_1, tr_98
- loc_en_US, sid_2005, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
- clientName_scbt
- bvseo_sdk, java_sdk, bvseo-4.0.0
- CLOUD, getReviews, 16ms
- REVIEWS, PRODUCT
goat anti-mouse IgG-HRP is rated
4.7 out of
5 by
99.
- y_2025, m_9, d_24, h_6
- bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
- cp_1, bvpage1
- co_hasreviews, tv_1, tr_98
- loc_en_US, sid_2005, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
- clientName_scbt
- bvseo_sdk, java_sdk, bvseo-4.0.0
- CLOUD, getAggregateRating, 105ms
- REVIEWS, PRODUCT
Santa Cruz Animal Health is a leading provider of animal health care products. ©2025 Santa Cruz Biotechnology Inc. All Rights Reserved.
Toll-Free: (800) 457-3801 - Terms & Conditions | Privacy Policy | Legal
“Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.
Toll-Free: (800) 457-3801 - Terms & Conditions | Privacy Policy | Legal
“Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.