Western blot analysis of CaMKI phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-CaMKI (C-2): sc-271988 (A,B) and CaMKI (H-8): sc-137225 (C,D). Antibody tested:
Lambda Phosphatase: sc-200312A
- available in 100,000 units in 0.25 ml (sc-200312) and 20,000 units in 0.05 ml (sc-200312A) sizes
- Bacteriophage λ, recombinant (E. coli)
- Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins; also active on phosphorylated histidine residues
- 1 mL of 10X Reaction buffer and 1 mL of 10X MnCl2 included with each size
- Lambda Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. The enzyme is inhibited by vanadate ions and can be heat inactivated at 65° C for 1 hour in the presence of 50 mM EDTA
- Purity: minimum 95% (SDS-PAGE) with no detectable protease, DNase, or RNase activity
- supplied as a solution containing approximately 400,000 units of protein per ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.01% BRIJ 35 and 50% glycerol
- Unit Definition: One unit will hydrolyze 1 nmole of p-nitophenyl phosphate per minute at 30° C and pH 7.5
QUICK LINKS
Datasheets and Protocols
SEE ALSO...
phosvitin Antibodies including preferred monoclonal antibody phosvitin Antibody (D-5)
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lambda Phosphatase | sc-200312A | 20,000 units | $130.00 | |||
Lambda Phosphatase | sc-200312 | 100,000 units in 0.25ml | $481.00 |