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Lambda Phosphatase : sc-200312

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  • available in 100,000 units in 0.25ml (sc-200312) and 20,000 units in 0.05ml (sc-200312A) sizes
  • Bacteriophage λ, recombinant (E. coli)
  • Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins; also active on phosphorylated histidine residues
  • 1 mL of 10X Reaction buffer and 1 mL of 10X MnCl2 included with each size
  • Lambda Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. The enzyme is inhibited by vanadate ions and can be heat inactivated at 65º C for 1 hour in the presence of 50 mM EDTA.
  • Purity: minimum 95% (SDS-PAGE) with no detectable protease, DNase, or RNase activity
  • supplied as a solution containing approximately 400,000 units of protein per ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.01% BRIJ 35 and 50% glycerol.
  • Unit Definition: One unit will hydrolyze 1 nmole of p-nitophenyl phosphate per minute at 30º C and pH 7.5
  • See 5 citations for Lambda Phosphatase
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Product NameCatalog #UnitPriceQtyAddFavorites
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116

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Lambda Phosphatase Data
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Western blot analysis of CaMKI phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-CaMKI (C-2): sc-271988 (A,B) and CaMKI (H-8): sc-137225 (C,D).
Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A,D), untreated human FAK transfected: sc-114600 (B,E) and lambda protein phosphatase treated human FAK transfected: sc-114600 (C,F) 293T whole cell lysates. Antibodies tested include p-FAK (Tyr 577)-R: sc-16665-R (A,B,C) and FAK (C-903): sc-932 (D,E,F).