NCK2 (8.8) Antibody sc-20020

santa cruz biotechnology, inc.

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NCK2 (8.8) Antibody: sc-20020	\| Datasheet

mouse monoclonal IgG_2b; 200 µg/ml
epitope mapping within amino acids 176-274 of NCK2 of human origin
recommended for detection of NCK1 and NCK2 of mouse and human origin by WB, IP, IF and IHC(P)

Additional NCK Antibodies ...

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Recommended Support Products:

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WB IP IF IHC(P)

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	NCK2	8440	2q12.2	NM_001004720, NM_001004722, NM_003581	O43639	604930
Mouse	Nck2	17974	1 B	NM_010879	NP_035009	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
NCK2 (8.8)	sc-20020	200 µg/ml	$279

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
U-937 Cell Lysate	sc-2239	500 µg/200 µl	$104
NCK2 (m): 293T Lysate	sc-121954	100µg/200µl	$205

NCK2 Background Information
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKå) and NCK2 (NCK∫), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-∫ (Tyr-1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.

NCK2 (8.8) Product Citations

See how others have used NCK2 (8.8): sc-20020 antibody and or NCK2 (8.8) antibody conjugates.

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NCK2 (8.8)

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NCK2 (8.8): sc-20020. Western blot analysis of NCK2 expression in U-937 whole cell lysate.

NCK2 (8.8): sc-20020. Immunofluorescence staining of methanol-fixed H4 cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (B) showing cytoplasmic staining.

NCK2 (8.8): sc-20020. Western blot analysis of NCK2 expression in U-937 whole cell lysate.

NCK2 (8.8): sc-20020. Western blot analysis of NCK2 expression in non-transfected: sc-117752 (A) and mouse NCK2 transfected: sc-121954 (B) 293T whole cell lysates.

NCK2 (8.8): sc-20020. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane staining of microvilli in glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

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NCK2 (8.8) Antibody: sc-20020