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Western blot analysis of CD13 expression in GBB whole cell lysate (A) and GBB whole cell lysate immunoprecipitated with CD
Western blot analysis of CD13 expression in GBB whole cell lysate (A) and GBB whole cell lysate immunoprecipitated with CD13 (BR2): sc-53970 (B) and detected with CD13 (3D8): sc-13536. Immunoprecipitation reagent used: Protein G PLUS-Agarose: sc-2002.
Western blot analysis of CD13 expression in GBB whole cell lysate (A) and GBB whole cell lysate immunoprecipitated with CD13 (BR2): sc-53970 (B) and detected with CD13 (3D8): sc-13536. Immunoprecipitation reagent used: Protein G PLUS-Agarose: sc-2002.

Protein G PLUS-Agarose: sc-2002

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Datasheets
  • provided as 0.5 ml agarose/2.0 ml; 100 reactions
  • suitable for use at 20 µl per immunoprecipitation reaction
  • pre-treated to reduce non-specific immunoglobulin binding
  • linked to Sepharose CL-6B
  • binding capacity is 10 mg of antibody per 1 ml of packed beads, settled not spun
  • strong binding to mouse IgG1, mouse IgG2a, IgG2b and IgG3, rat IgG2a and IgG2c, human IgG1, IgG2, IgG3 and IgG4, rabbit, bovine and ovine IgG
  • weak to medium binding to rat IgG1, rat IgG2b, human IgM, IgA and IgE, porcine, canine, feline and guinea pig IgG
  • pre-washed and ready to use

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Description
Datasheets and Protocols

SEE ALSO...

Molecular purification methods such as immunoprecipitation (IP), co-IP and chromatin IP are important tools in studying lowly expressed proteins, protein-protein interactions or protein-nucleic acid interactions. Santa Cruz Biotechnology offers a comprehensive array of research support products for purification techniques. Protein G Agarose is a recommended specialty reagent used for isolating immunoglobulins from mammalian species. Protein G is an IgG binding protein expressed in Streptococcal bacteria much like Protein A but with differing binding specificities. Protein G binds to the Fc region of most mammalian immunoglobulins making it a powerful tool for antibody purification. Protein G Agarose is a recombinant Protein G type 3 that is linked to a high-quality Agarose resin, CL-6B, and together makes a superior and premium product which produces high purity and purification yield of IgGs from mammalian samples. Protein G Agarose is used with a wider range of IgG subclasses of human, mouse and rat serum than Protein A.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Protein G PLUS-Agarose References:

  1. CM1 ligation initiates apoptosis in a caspase 8-dependent manner in Ramos cells and in a mitochondria-controlled manner in Raji cells.  |  Kim, D., et al. 2002. Hum Immunol. 63: 576-87. PMID: 12072193
  2. Heterologous expression and purification of SpaB involved in subtilin biosynthesis.  |  Xie, L., et al. 2002. Biochem Biophys Res Commun. 295: 952-7. PMID: 12127987
  3. KRIT1, a gene mutated in cerebral cavernous malformation, encodes a microtubule-associated protein.  |  Gunel, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 10677-82. PMID: 12140362
  4. Transient Activation and Tyrosine Phosphorylation of a Protein Kinase in Tobacco Cells Treated with a Fungal Elicitor.  |  Suzuki, K. and Shinshi, H. 1995. Plant Cell. 7: 639-647. PMID: 12242379
  5. Analysis of the membrane topology for transmembrane domains 7-12 of the human reduced folate carrier by scanning cysteine accessibility methods.  |  Cao, W. and Matherly, LH. 2004. Biochem J. 378: 201-6. PMID: 14602046
  6. Interleukin-17F signaling requires ubiquitination of interleukin-17 receptor via TRAF6.  |  Rong, Z., et al. 2007. Cell Signal. 19: 1514-20. PMID: 17346928
  7. Tau binds both subunits of calcineurin, and binding is impaired by calmodulin.  |  Yu, DY., et al. 2008. Biochim Biophys Acta. 1783: 2255-61. PMID: 18639592
  8. IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.  |  Rong, Z., et al. 2009. Cell Res. 19: 208-15. PMID: 19079364
  9. Targeted overexpression of EZH2 in the mammary gland disrupts ductal morphogenesis and causes epithelial hyperplasia.  |  Li, X., et al. 2009. Am J Pathol. 175: 1246-54. PMID: 19661437
  10. Rictor regulates FBXW7-dependent c-Myc and cyclin E degradation in colorectal cancer cells.  |  Guo, Z., et al. 2012. Biochem Biophys Res Commun. 418: 426-32. PMID: 22285861
  11. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75.  |  D′Eletto, M., et al. 2018. Cell Rep. 25: 3573-3581.e4. PMID: 30590033
  12. G protein beta gamma subunits stimulate phosphorylation of Shc adapter protein.  |  Touhara, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 9284-7. PMID: 7568118
  13. Role of p21 RAS in p210 bcr-abl transformation of murine myeloid cells.  |  Mandanas, RA., et al. 1993. Blood. 82: 1838-47. PMID: 7691239
  14. Acute inflammatory injury in the lung precipitated by oxidant stress induces fibroblasts to synthesize and release transforming growth factor-alpha.  |  Vivekananda, J., et al. 1994. J Biol Chem. 269: 25057-61. PMID: 7929192

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Protein G PLUS-Agarose

sc-2002
2 ml
$63.00

In the introduction of sc-2002, it says "binding capacity is 10 mg of antibody per 1 ml of packed beads", is this true? that means 1 ul of the beads can bind 10 ug of antibody. I believe the reasonable range should be 10-20 ul of beads per ug of antibody

Asked by: YZHAN60
Thank you for your question. Please contact technical service by emailing scbt@scbt.com
Answered by: BlakeJ
Date published: 2023-08-21

en sc-525409 recomiendan el uso de la proteína g plus para la realización de WB en tejidos animales, ¿se usa el mismo protocolo que en IP?

Asked by: Alex08
Thank you for your product review. We are sorry to hear that this antibody did not perform as expected. We want you to be successful in your research, so please contact our Technical Service Department by phone, e-mail or live chat.
Answered by: Technical Service
Date published: 2022-10-06

Hello there, the provided protocol for IP mentioned elution is achieved via "boil". So in the "boil" condition, whether or not the agarose beads would melt?

Asked by: Jacksonnn
Thank you for your question. The agarose beads will not melt under these conditions. They are stable under this high temperature. We recommend to "boil" at 95C for 5 minutes. This is the recommended method if you are using SDS buffer for elution. High temperature denatures protein and promotes denaturation by SDS.
Answered by: Technical Support
Date published: 2020-05-18

Hi there, I have a paper which mention protein G-Sepharose from Santa Cruz Biothechnology (it does not say the catalog number/ref number). When I googled it I got this PROTEIN G PLUS-AGAROSE sc2002. Could you confirm whether it is a right product for IP?

Asked by: Zsudy
Thank you for your question. Yes, sc-2002 is designed to be used for IP applications, so it is recommended for IP use.
Answered by: Tech Service
Date published: 2019-10-28

Which step should I use sc-2002 at, during western blot?

Asked by: yeji
Thank you for your question. You can find our recommended protocol for Immunoprecipitation/Western Blotting by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoprecipitation-western-blots Protein G PLUS-Agarose is used in the preclearing step of the cell lysate together with a control antibody, which removes proteins from the lysate that would cause unspecific results in later steps. If you are not using a primary antibody agarose conjugate you will also have to use Protein G PLUS-Agarose when incubating your cell lysate with the specific antibody. For further assistance, please get in touch with our technical service.
Answered by: Technical Support Europe
Date published: 2024-08-12
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Rated 5 out of 5 by from Immunoprecipitation experiments (for HA IP)Protein G Plus-agarose is always the best for immunoprecipiattion experiments
Date published: 2025-02-04
Rated 5 out of 5 by from It worksI bought this item about a month ago. I tried it and it seems to be working.
Date published: 2020-07-24
Rated 5 out of 5 by from Good dealIt is less expensive and works for Immunoprecipitation with mouse and rabbit Ig.
Date published: 2019-08-24
Rated 5 out of 5 by from Works Great!We continue to buy this over and over again, and we're so happy with the results!
Date published: 2019-04-26
Rated 5 out of 5 by from Works wellWe have already bought it twice and works well. A+
Date published: 2018-03-20
Rated 5 out of 5 by from Great for immunoprecipitationExcellent for immunoprecipitation with mouse antibodies. We have purchased this four times already, and it worked everytime with just 50 ul per sample.
Date published: 2018-02-02
Rated 4 out of 5 by from BuenoFunciona muy bien en inmunoprecipitaciones utilizando extractos celulares totales
Date published: 2018-01-08
Rated 5 out of 5 by from excellent product for IPI used this product many times, it worked very well.
Date published: 2017-02-04
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Protein G PLUS-Agarose is rated 5.0 out of 5 by 24.
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