Protein A-Agarose: sc-2001
- provided as 0.5 ml agarose/2.0 ml; 100 reactions
- suitable for use at 20 µl per immunoprecipitation reaction
- pre-treated to reduce non-specific immunoglobulin binding
- linked to a high-quality and well established Sepharose resin
- binding capacity is 10 mg of antibody per 1 ml of packed beads, settled not spun
- strong binding to mouse IgG2a, IgG2b and IgG3, rat IgG2c, human IgG1, IgG2, IgG4, rabbit, guinea pig, porcine, canine and feline IgG
- weak to medium binding to mouse and rat IgG1, and bovine, ovine and equine IgG
- pre-washed and ready to use
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SEE ALSO...
Protein A-Agarose is a critical tool in biochemical research, particularly for the purification of immunoglobulins, mainly Immunoglobulin G (IgG), from complex biological mixtures. This reagent consists of Protein A, derived from the cell wall of *Staphylococcus aureus*, which has a high affinity for the Fc region of IgGs across many species, covalently attached to agarose beads. The agarose provides a stable, inert support that facilitates high-throughput and gentle purification processes. Protein A-Agarose′s primary mechanism involves selectively binding IgGs, allowing other proteins and impurities to be washed away, followed by the elution of the IgGs under conditions that disrupt their interaction with Protein A. This method is extensively used in various research applications, including antibody isolation for Western blotting, immunoprecipitation, and ELISA, enhancing the study of protein interactions, immune responses, and disease mechanisms. Its ability to provide quick, efficient, and high-purity purification makes Protein A-Agarose an indispensable resource in laboratories focusing on molecular biology, immunology, and related fields.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Protein A-Agarose | sc-2001 | 2 ml | $44.00 |