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home » Synthesis and Degradation » NOS Antibody / NOS Antibodies » p-NOS3 (Thr 495): sc-19827

p-NOS3 (Thr 495) Antibody: sc-19827

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-19827-R
  • recommended for detection of NOS3 of mouse, rat and human origin phosphorylated at Thr 495 by WB, IF and ELISA
  • blocking peptide, sc-19827 P; also reactive with additional species, including equine, canine, bovine and porcine
 
Additional NOS Antibodies ...
 
Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IF   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NOS3 4846 7q36.1 NM_000603 P29474
 601367
Mouse Nos3 18127 5 A3 P70313
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-NOS3 (Thr 495) sc-19827 200 µg/ml $279
p-NOS3 (Thr 495) P sc-19827 P
(peptide)
 100 µg/0.5 ml $61
p-NOS3 (Thr 495)-R sc-19827-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100,000 units $462
Lambda Phosphatase sc-200312A 20,000 units $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NOS3 siRNA (h) sc-36093 10 µM $258
NOS3 siRNA (m) sc-36094 10 µM $258
NOS3 (h)-PR sc-36093-PR 10 µM $23
NOS3 (m)-PR sc-36094-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NOS3 shRNA Plasmid (h) sc-36093-SH 20 µg $520
NOS3 shRNA Plasmid (m) sc-36094-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NOS3 shRNA (h) Lentiviral Particles sc-36093-V 200 µl $625
NOS3 shRNA (m) Lentiviral Particles sc-36094-V 200 µl $625
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ECV304 Cell Lysate sc-2269 500 µg/200 µl $104

NOS3 Background Information
Nitric oxide (NO), produced by the endothelial NO synthase (NOS3), is a fundamental determinant of cardiovascular homeostasis that maintains system blood pressure, vascular remodeling and angiogenesis. NOS3 is stimulated, in a phosphatidylinositol 3-kinase (PI 3-kinase)-dependent fashion, by treatment of endothelial cells with insulin-like growth factor-1 and vascular endothelial growth factor (VEGF). The serine/ threonine protein kinase Akt/PKB is an important downstream target of PI 3-kinase, regulating VEGF-stimulated endothelial cell survival. NOS3 activation via phosphorylation of serine 1177 by Akt/PKB is necessary and sufficient for VEGF-mediated endothelial cell migration. Therefore, Akt/PKB can directly phosphorylate NOS3 on serine 1177, activating the enzyme and leading to NO production.

p-NOS3 (Thr 495)
Click on image to enlarge
sc-19827
Western blot analysis of NOS3 phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) HUV-EC-C whole cell lysates. Antibodies tested include p-NOS3 (Thr 495)-R: sc-19827-R (A,B) and NOS3 (C-20): sc-654 (C,D).
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