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FAS-L Antibody (NOK-1): sc-19681

 |  Datasheet

(Based on data analysis)

  • FAS-L Antibody (NOK-1) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against L5178Y mouse T lymphoma cells expressing recombinant human FAS-L
  • recommended for detection of FAS-L of human origin by IP, IF and FCM; not recommended for WB
  • fluorescein (sc-19681 FITC) and phycoerythrin (sc-19681 PE) conjugates for FCM, 100 tests
  • also provided azide-free for neutralizing the cytotoxic effects of FAS-L, sc-19681 L, 200 µg/0.1 ml
  • See 8 citations for FAS-L Antibody (NOK-1)
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
FAS-L Antibody (NOK-1) sc-19681 200 µg/ml $279
FAS-L Antibody (NOK-1) FITC sc-19681 FITC 100 tests in 2 ml $292
FAS-L Antibody (NOK-1) L sc-19681 L 200 µg/0.1 ml $279
FAS-L Antibody (NOK-1)-PE sc-19681 PE 100 tests in 2 ml $303
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
FAS-L siRNA (h) sc-29313 10 µM $258
FAS-L (h)-PR sc-29313-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
FAS-L shRNA Plasmid (h) sc-29313-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
FAS-L shRNA (h) Lentiviral Particles sc-29313-V 200 µl $625
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Hep G2 Cell Lysate sc-2227 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human FASLG 356 1q24.3 NM_000639 P48023
 152700
 

Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immunosurveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity.

FAS-L Antibody (NOK-1) Product Citations
See how others have used FAS-L Antibody (NOK-1) and or FAS-L Antibody (NOK-1) conjugates.


8 total citations
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FAS-L Antibody (NOK-1) Data
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sc-19681
FAS-L (NOK-1): sc-19681. Western blot analysis of FAS-L expression in K-562 (A) and HL-60 (B) whole cell lysates immunoprecipitated with FAS-L (NOK-1): sc-19681 and detected with FAS-L (C-178): sc-6237.
sc-19681
FAS-L (NOK-1): sc-19681. Western blot analysis of FAS-L expression in K-562 (A) and HL-60 (B) whole cell lysates immunoprecipitated with FAS-L (NOK-1): sc-19681 and detected with FAS-L (N-20): sc-834.
sc-19681
FAS-L (NOK-1): sc-19681. Indirect FCM analysis of L5178Y-human FAS-L stable transfectant cell line (B) and non-transfected L5178Y cells (A). Cells were treated with metalloproteinase inhibitor BB94 and stained with FAS-L (NOK-1) (orange), followed by PE-conjugated goat anti-mouse IgG. Clear line histograms represent the isotype control, normal mouse IgG1. Data kindly provided by Dr. Hideo Yagita at Juntendo University School of Medicine.
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