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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of E2F-1 of human origin
- recommended for detection of E2F-1 and, to a lesser extent, E2F-2 and E2F-3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-193 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-193 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
E2F1 |
1869 |
20q11.22 |
NM_005225 |
Q01094
|
189971 |
| Mouse |
E2f1 |
13555 |
2 H1 |
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Q61501
|
N/A |
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E2F-1 Background Information
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.
| E2F-1 (C-20) Product Citations |
See how others have used E2F-1 (C-20): sc-193 antibody and or E2F-1 (C-20) antibody conjugates.
214 total citations Loading citations.
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E2F-1 (C-20)
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E2F-1 (C-20): sc-193. Western blot analysis of BALB/3T3 cells transfected with either y-CRE (A) or y-CRE/E2F (B).
ChIP analysis of E2F transcription factor binding to the c-Myc promoter in NBS cells. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced with permission from Master, et al. 2001. Mol. Cell. Biol. 21: 6006-6016. Extracts of 293 cells transiently transfected with plasmid containing the murine carboxylesterase promoter were subjected to ChIP analysis using the following E2F antibodies: E2F-1 (C-20) X: sc-193 X (A), E2F-2 (C-20) X: sc-633 X (B), E2F-3 (C-18) X: sc-878 X (C), E2F-4 (C-20) X: sc-866 X (D), E2F-5 (E-19) X: sc-999 X (E), E2F-6 (E-20) X: sc-8366 X (F). Controls: normal rabbit IgG: sc-2027 (G), no antibody (H), input DNA (I) and mock (J). Data kindly provided by J. L. Lavrrar and reproduced from J. Biol. Chem. 279: 46343-46349, 2004, with permission from the American Society for Biochemistry and Molecular Biology.
ChIP analysis of E2F transcription factor binding to the oriP (I.) and c-Myc (II.) promoters in Raji cells as a function of the cell cycle. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced from Master, et al., 2001. Mol. Cell. Biol. 21: 6006-6016. E2F-1 (C-20): sc-193. Western blot analysis of E2F-1 expression in non-transfected: sc-117752 (A) and human E2F-1 transfected: sc-116659 (B) 293T whole cell lysates. E2F-1 (C-20): sc-193. Western blot analysis of E2F-1 expression in non-transfected: sc-117752 (A) and human E2F-1 transfected: sc-116137 (B) 293T whole cell lysates. E2F-1 (C-20): sc-193. Western blot analysis of E2F-1 expression in non-transfected: sc-117752 (A) and mouse E2F-1 transfected: sc-126755 (B) 293T whole cell lysates. E2F-1 (C-20): sc-193. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
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