goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-17922-R
epitope corresponding to a short amino acid sequence containing phosphorylated Ser 345 of Chk1 of human origin
recommended for detection of Ser 345 phosphorylated Chk1 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
Chk1 Background Information Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins (1). Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phospho-rylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis (2–5). Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues (6). It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1 (3). Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU) (7). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (7).
p-Chk1 (Ser 345) Product Citations
See how others have used p-Chk1 (Ser 345): sc-17922 antibody and or p-Chk1 (Ser 345) antibody conjugates.
