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home » Transcription Regulators » ETS-Related Protein Net Antibody / ETS-Related Protein Net Antibodies » Net (C-20): sc-17860

Net (C-20) Antibody: sc-17860

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml
  • epitope mapping at the C-terminus of Net of human origin
  • recommended for detection of Net of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • blocking peptide, sc-17860 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-17860 X, 200 µg/0.1 ml
 
Additional ETS-Related Protein Net Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ELK3 2004 12q23.1 NM_005230 P41970
 600247
Mouse Elk3 13713 10 C2 P41971
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Net (C-20) sc-17860 200 µg/ml $279
Net (C-20) P sc-17860 P
(peptide)
 100 µg/0.5 ml $61
Net (C-20) X sc-17860 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Net siRNA (h) sc-37867 10 µM $258
Net siRNA (m) sc-37868 10 µM $258
Net (h)-PR sc-37867-PR 10 µM $23
Net (m)-PR sc-37868-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Net shRNA Plasmid (h) sc-37867-SH 20 µg $520
Net shRNA Plasmid (m) sc-37868-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Net shRNA (h) Lentiviral Particles sc-37867-V 200 µl $625
Net shRNA (m) Lentiviral Particles sc-37868-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
rat lung extract sc-2396 500 µg/200 µl $104
mouse lung extract sc-2390 500 µg/200 µl $104

Net Background Information
Ras signaling is mediated in part by transcription factors, which belong to one of the largest families of signal-dependent transcriptional regulators, the Ets gene family. One member of the Ets gene family, Net (also designated ERP and SAP-2), shares various properties with Ets factors Elk1 and SAP-1. Like Ets factors Elk-1 and SAP-1, Net binds to Ets DNA motifs through the Ets domain and forms ternary complexes with the serum response factor SRF on the Fos serum response element SRE. Net contains two nuclear localization signals, one in the Ets domain and one corresponding to the D box, as well as a nuclear export signal in the conserved Ets DNA binding domain. Net is exported from the nucleus in response to stress stimuli transduced through the JNK pathway. ERK and p38 bind to the D box of Net to allow phosphoryl-ation of the adjacent C-terminal C-domain, which, in combination with the D box, is required for transcription activation by Ras. However, the binding of JNK to the J Box results in phosphorylation of the adjacent export motif, which is important for Net export from the nucleus. Therefore, Net acts as a transcriptional repressor that is converted into an activator by Ras/ERK signaling and is regulated by nuclear-cytoplasmic shuttling in response to specific signaling pathways.

Net (C-20) Product Citations
See how others have used Net (C-20): sc-17860 antibody and or Net (C-20) antibody conjugates.


3 total citations
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Net (C-20)
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sc-17860
Net (C-20): sc-17860. Western blot analysis of Net expression in mouse lung tissue extract.
sc-17860
Net (C-20): sc-17860. Western blot analysis of Net expression in rat lung tissue extract.
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