Sp1 Antibody (E-3): sc-17824
- Sp1 Antibody (E-3) is a mouse monoclonal IgG2a, cited in 136 publications, provided at 200 µg/ml
- raised against amino acids 121-345 mapping near the N-terminus of Sp1 of human origin
- Anti-Sp1 Antibody (E-3) is recommended for detection of Sp1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Sp1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-17824 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Sp1 Antibody (E-3) for WB applications. This reagent is now offered in a bundle with Sp1 Antibody (E-3) (see ordering information below).
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Sp1 Antibody (E-3) is a mouse monoclonal IgG2a antibody that detects Specificity Protein 1 (Sp1) in mouse, rat, and human species by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Sp1 antibody (E-3) is raised against amino acids 121-345 near the N-terminus of human-origin Sp1, also known as Transcription Factor Sp1, which is a critical transcription factor involved in gene expression regulation. Sp1 (E-3) monoclonal antibody recognizes a protein characterized by three C2H2-type zinc finger motifs that facilitate high-affinity binding to GC-rich motifs in DNA, known as GC boxes, thereby influencing the transcription of genes essential for cellular processes such as growth, differentiation, and apoptosis. Sp1 (E-3) antibody targets a protein predominantly localized in the nucleus, where Sp1 directly interacts with promoter regions of target genes to modulate transcriptional activity. Dysregulation of Sp1 has been linked to several diseases, including cancer and neurodegenerative disorders, underscoring the importance of understanding regulatory mechanisms. Anti-Sp1 antibody (E-3) enables researchers to effectively detect and analyze Sp1 expression and localization across different biological contexts, enhancing our comprehension of its pivotal role in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sp1 Antibody (E-3) References:
- Transcriptional factor specificity protein 1 (SP1) promotes the proliferation of glioma cells by up-regulating midkine (MDK). | Luo, J., et al. 2015. Mol Biol Cell. 26: 430-9. PMID: 25428991
- Arsenic suppresses GDF1 expression via ROS-dependent downregulation of specificity protein 1. | Gao, X., et al. 2021. Environ Pollut. 271: 116302. PMID: 33360347
- Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues. | Shi, CS., et al. 2021. Int J Ophthalmol. 14: 223-227. PMID: 33614450
- Specificity Protein 1: A Protein With a Two-Sided Role in Ischemic Stroke. | Yu, Q., et al. 2021. Front Cell Neurosci. 15: 757670. PMID: 34970121
- Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension. | DeVallance, ER., et al. 2022. Antioxidants (Basel). 12: PMID: 36670936
- The Role of Specificity Protein 1 (SP1) in Bladder Cancer Progression through PTEN-Mediated AKT/mTOR Pathway. | Chen, Z. 2023. Urol Int. 107: 848-856. PMID: 37666229
- Deficiency of Transcription Factor Sp1 Contributes to Hypertrophic Cardiomyopathy. | Zhang, F., et al. 2024. Circ Res. 134: 290-306. PMID: 38197258
- Transcription factor Sp1 transcriptionally enhances GSDME expression for pyroptosis. | Pan, J., et al. 2024. Cell Death Dis. 15: 66. PMID: 38238307
- Glucocorticoid receptor and specificity protein 1 (Sp1) or Sp3, but not the antibiotic Mithramycin A, stimulates human alphaherpesvirus 1 (HSV-1) replication. | El-Mayet, FS., et al. 2024. Antiviral Res. 225: 105870. PMID: 38556059
- Role of Specificity Protein 1 (SP1) in Cardiovascular Diseases: Pathological Mechanisms and Therapeutic Potentials. | Ding, J., et al. 2024. Biomolecules. 14: PMID: 39062521
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sp1 Antibody (E-3) | sc-17824 | 200 µg/ml | $316.00 | |||
Sp1 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-525493 | 200 µg Ab; 10 µg BP | $354.00 | |||
Sp1 Antibody (E-3) X | sc-17824 X | 200 µg/0.1 ml | $316.00 | |||
Sp1 Antibody (E-3) AC | sc-17824 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Sp1 Antibody (E-3) HRP | sc-17824 HRP | 200 µg/ml | $316.00 | |||
Sp1 Antibody (E-3) FITC | sc-17824 FITC | 200 µg/ml | $330.00 | |||
Sp1 Antibody (E-3) PE | sc-17824 PE | 200 µg/ml | $343.00 | |||
Sp1 Antibody (E-3) Alexa Fluor® 488 | sc-17824 AF488 | 200 µg/ml | $357.00 | |||
Sp1 Antibody (E-3) Alexa Fluor® 546 | sc-17824 AF546 | 200 µg/ml | $357.00 | |||
Sp1 Antibody (E-3) Alexa Fluor® 594 | sc-17824 AF594 | 200 µg/ml | $357.00 | |||
Sp1 Antibody (E-3) Alexa Fluor® 647 | sc-17824 AF647 | 200 µg/ml | $357.00 | |||
Sp1 Antibody (E-3) Alexa Fluor® 680 | sc-17824 AF680 | 200 µg/ml | $357.00 | |||
Sp1 Antibody (E-3) Alexa Fluor® 790 | sc-17824 AF790 | 200 µg/ml | $357.00 |