Sp1 Antibody (E-3): sc-17824

Sp1 Antibody (E-3) is a mouse monoclonal IgG2a antibody that detects Specificity Protein 1 (Sp1) in mouse, rat, and human species by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Sp1 antibody (E-3) is raised against amino acids 121-345 near the N-terminus of human-origin Sp1, also known as Transcription Factor Sp1, which is a critical transcription factor involved in gene expression regulation. Sp1 (E-3) monoclonal antibody recognizes a protein characterized by three C2H2-type zinc finger motifs that facilitate high-affinity binding to GC-rich motifs in DNA, known as GC boxes, thereby influencing the transcription of genes essential for cellular processes such as growth, differentiation, and apoptosis. Sp1 (E-3) antibody targets a protein predominantly localized in the nucleus, where Sp1 directly interacts with promoter regions of target genes to modulate transcriptional activity. Dysregulation of Sp1 has been linked to several diseases, including cancer and neurodegenerative disorders, underscoring the importance of understanding regulatory mechanisms. Anti-Sp1 antibody (E-3) enables researchers to effectively detect and analyze Sp1 expression and localization across different biological contexts, enhancing our comprehension of its pivotal role in health and disease.

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Sp1 Antibody (E-3) References:

1. Transcriptional factor specificity protein 1 (SP1) promotes the proliferation of glioma cells by up-regulating midkine (MDK).  |  Luo, J., et al. 2015. Mol Biol Cell. 26: 430-9. PMID: 25428991
2. Arsenic suppresses GDF1 expression via ROS-dependent downregulation of specificity protein 1.  |  Gao, X., et al. 2021. Environ Pollut. 271: 116302. PMID: 33360347
3. Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues.  |  Shi, CS., et al. 2021. Int J Ophthalmol. 14: 223-227. PMID: 33614450
4. Specificity Protein 1: A Protein With a Two-Sided Role in Ischemic Stroke.  |  Yu, Q., et al. 2021. Front Cell Neurosci. 15: 757670. PMID: 34970121
5. Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.  |  DeVallance, ER., et al. 2022. Antioxidants (Basel). 12: PMID: 36670936
6. The Role of Specificity Protein 1 (SP1) in Bladder Cancer Progression through PTEN-Mediated AKT/mTOR Pathway.  |  Chen, Z. 2023. Urol Int. 107: 848-856. PMID: 37666229
7. Deficiency of Transcription Factor Sp1 Contributes to Hypertrophic Cardiomyopathy.  |  Zhang, F., et al. 2024. Circ Res. 134: 290-306. PMID: 38197258
8. Transcription factor Sp1 transcriptionally enhances GSDME expression for pyroptosis.  |  Pan, J., et al. 2024. Cell Death Dis. 15: 66. PMID: 38238307
9. Glucocorticoid receptor and specificity protein 1 (Sp1) or Sp3, but not the antibiotic Mithramycin A, stimulates human alphaherpesvirus 1 (HSV-1) replication.  |  El-Mayet, FS., et al. 2024. Antiviral Res. 225: 105870. PMID: 38556059
10. Role of Specificity Protein 1 (SP1) in Cardiovascular Diseases: Pathological Mechanisms and Therapeutic Potentials.  |  Ding, J., et al. 2024. Biomolecules. 14: PMID: 39062521

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sp1 Antibody (E-3) has 136 citations in a variety of scientific publications.

Primary antibodies like Anti-Sp1 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sp1 Antibody (E-3) Specifications

Alternate Product Name: Sp1

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 121-345

Epitope Target: Sp1

Epitope Species: human

Product Reactivity/Detection: Sp1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 136 citations in various scientific publications

Available Conjugates/Forms for Sp1 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Sp1 antibody is Sp1 Antibody (1C6): sc-420.