SYP/Synaptophysin Antibody (D-4): sc-17750

SYP Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects synaptophysin (SYP) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SYP antibody (D-4) is available in non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Synaptophysin is an integral membrane protein found mainly in neurons and endocrine cells, playing a vital role in synaptic vesicle formation and recycling. SYP features N-glycosylation and forms hexameric complexes that contribute to large conductance channel formation. SYP contains four transmembrane domains and functions similarly to gap junctions for cell-to-cell communication. Membrane cholesterol specifically interacts with SYP, influencing vesicle formation and dynamics. Synaptobrevin (VAMP) forms a complex with SYP that increases during neuronal development, though not essential for exocytosis. Brain myosin V′s tail domain creates a stable complex with synaptobrevin II and SYP, which dissociates upon calcium influx during depolarization, demonstrating dynamic protein interactions in synaptic function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SYP/Synaptophysin Antibody (D-4) References:

1. The synaptophysin-synaptobrevin complex: a hallmark of synaptic vesicle maturation.  |  Becher, A., et al. 1999. J Neurosci. 19: 1922-31. PMID: 10066245
2. Lipids, lipid modification and lipid-protein interaction in membrane budding and fission--insights from the roles of endophilin A1 and synaptophysin in synaptic vesicle endocytosis.  |  Huttner, WB. and Schmidt, A. 2000. Curr Opin Neurobiol. 10: 543-51. PMID: 11084315
3. Endocytosis of the synaptic vesicle protein, synaptophysin, requires the COOH-terminal tail.  |  Linstedt, AD. and Kelly, RB. 1991. J Physiol (Paris). 85: 90-6. PMID: 1757893
4. Torpedo synaptophysin: evolution of a synaptic vesicle protein.  |  Cowan, D., et al. 1990. Brain Res. 509: 1-7. PMID: 2106365
5. Adverse effects of SYP-3343 on zebrafish development via ROS-mediated mitochondrial dysfunction.  |  Chen, G., et al. 2022. J Hazard Mater. 437: 129382. PMID: 35749898
6. Imp and Syp mediated temporal patterning of neural stem cells in the developing Drosophila CNS.  |  Islam, IM. and Erclik, T. 2022. Genetics. 222: PMID: 35881070
7. Circ-Vps41 positively modulates Syp and its overexpression improves memory ability in aging mice.  |  Li, Y., et al. 2022. Front Mol Neurosci. 15: 1037912. PMID: 36533129
8. SYP-3343 drives abnormal vascularization in zebrafish through regulating endothelial cell behavior.  |  Chen, G., et al. 2023. Food Chem Toxicol. 174: 113671. PMID: 36796616
9. The role of Imp and Syp RNA-binding proteins in precise neuronal elimination by apoptosis through the regulation of transcription factors.  |  Guan, W., et al. 2024. Elife. 12: PMID: 39364747
10. Vesicle-associated membrane protein and synaptophysin are associated on the synaptic vesicle.  |  Calakos, N. and Scheller, RH. 1994. J Biol Chem. 269: 24534-7. PMID: 7929121
11. Synthesis of the mammalian synaptic vesicle protein synaptophysin in insect cells: a model for vesicle biogenesis.  |  Leimer, U., et al. 1996. Exp Cell Res. 224: 88-95. PMID: 8612696
12. Brain myosin V is a synaptic vesicle-associated motor protein: evidence for a Ca2+-dependent interaction with the synaptobrevin-synaptophysin complex.  |  Prekeris, R. and Terrian, DM. 1997. J Cell Biol. 137: 1589-601. PMID: 9199173

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SYP/Synaptophysin Antibody (D-4) has 177 citations in a variety of scientific publications.

Primary antibodies like Anti-SYP/Synaptophysin Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SYP/Synaptophysin Antibody (D-4) Specifications

Alternate Product Name: Synaptophysin

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 221-313

Epitope Target: SYP

Epitope Species: human

Product Reactivity/Detection: SYP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 177 citations in various scientific publications

Available Conjugates/Forms for SYP/Synaptophysin Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin