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p-ERK 1/2 Antibody : sc-16982

 |  Datasheet

(Based on data analysis)

  • p-ERK 1/2 Antibody (Thr 202/Tyr 204) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-16982-R
  • recommended for detection of ERK 1 phosphorylated at Thr 202 and Tyr 204 and ERK 2 phosphorylated at Thr 185 and Tyr 187 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • blocking peptide, sc-16982 P
  • See 76 citations for p-ERK 1/2 Antibody
 

See additional ERK Antibodies including ERK, ERK 1, ERK 2, ERK 3, ERK 4 and ERK 5.

Also see ERK 1 Inhibitors, ERK 2 Inhibitors, ERK 1 Activators, ERK 2 Activators, ERK 1 Substrates or ERK 2 Substrates for functional analysis of cellular responses to ERK 1 and ERK 2.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-ERK 1/2 Antibody (Thr 202/Tyr 204) sc-16982 200 µg/ml $279
p-ERK 1/2 (Thr 202/ Tyr 204) sc-16982 P
(peptide)
100 µg/0.5 ml $61
p-ERK 1/2 Antibody (Thr 202/Tyr 204/Thr 185/Tyr 187)-R sc-16982-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa + TNFα Cell Lysate sc-2228 500 µg/200 µl $104
ERK 1 (m): 293T Lysate sc-126806 100 µg/200 µl $205
HeLa+UV irradiated Cell Lysate sc-2221 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAPK3 5595 16p11.2 NM_001040056, NM_002746 P27361
601795
Mouse Mapk1 26413 16 A3 P63085
N/A
Mouse Mapk3 26417 7 F3 Q63844
N/A
 


The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively (1–3). ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif (4,5). Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation (6). The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop (6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (4,5). Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1 (5). The ERK family has three additional members: ERK 3, ERK 5 and ERK 6 (7,8).

p-ERK 1/2 Antibody Product Citations
See how others have used p-ERK 1/2 Antibody and or p-ERK 1/2 Antibody conjugates.


76 total citations
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p-ERK 1/2 Antibody Data
Click on image to enlarge
Western blot analysis of phosphorylated ERK 1/2 expression in untreated Jurkat (A) and PMA-treated Jurkat (B,C) whole cell lysates. Blots were probed with p-ERK 1/2 (Thr 202/ Tyr 204): sc-16982 (A,B) and p-ERK 1/2 (Thr 202/ Tyr 204): sc-16982 preincubated with its cognate phosphorylated peptide (C).
Western blot analysis of phosphorylated ERK 1/2 expression in untreated Jurkat (A), PMA-treated Jurkat (B,E), untreated NIH/3T3 (C) and PDGF-treated NIH/3T3 (D,F) whole cell lysates. Blots were probed with p-ERK 1/2 (Thr 202/ Tyr 204)-R: sc-16982-R (A,B,C,D) and p-ERK 1/2 (Thr 202/ Tyr 204)-R: sc-16982-R preincubated with its cognate phosphorylated peptide (E,F).
p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R. Western blot analysis of ERK 1/2 phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R. Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Hela whole cell lysates.
p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R. Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and Raf Kinase Inhibitor V (sc-222241) treated (B) SK-MEL-28 whole cell lysates. Note inhibition of ERK 1/2 phosphorylated in lane B.
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and L-779,450 (sc-204046) treated (B) SK-MEL-28 whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R (A,B). Note inhibition of ERK 1/2 phosphorylation by L-779,450 in lane B.
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and L-779,450 (sc-204046) treated (B) SW480 whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R (A,B). Note inhibition of ERK 1/2 phosphorylation by L-779,450 in lane B.
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and PD 0325901 (sc-205427) treated (B) SK-MEL-28 whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R (A,B). Note inhibition of ERK 1/2 phosphorylation by PD 0325901 in lane B.
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and PD 0325901 (sc-205427) treated (B) SW480 whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R (A,B). Note inhibition of ERK 1/2 phosphorylation by PD 0325901 in lane B.
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and PD 0325901 (sc-205427) treated (B) SK-MEL-28 whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 202/Tyr 204)-R: sc-16982-R (A,B). Note inhibition of ERK 1/2 phosphorylation by PD 0325901 in lane B.
p-ERK 1/2 (Thr 202/Tyr 204)-R : sc-16982-R. Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages.
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