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p-ERK 1/2 Antibody (Thr 177)-R: sc-16981-R

 |  Datasheet

(Based on data analysis)

  • p-ERK 1/2 (Thr 177)-R Antibody is a rabbit polyclonal IgG provided at 200 µg/ml
  • recommended for detection of Thr 177 phosphorylated ERK 1 and ERK 2 of mouse, rat and human origin by WB, IP, IF and ELISA
  • blocking peptide, sc-16981 P
 

See additional ERK Antibodies including ERK, ERK 1, ERK 2, ERK 3, ERK 4 and ERK 5.

Also see ERK 1 Inhibitors, ERK 2 Inhibitors, ERK 1 Activators, ERK 2 Activators, ERK 1 Substrates or ERK 2 Substrates for functional analysis of cellular responses to ERK 1 and ERK 2.


Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IP   IF  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-ERK 1/2 (Thr 177) P sc-16981 P
(peptide)
 100 µg/0.5 ml $61
p-ERK 1/2 (Thr 177)-R Antibody sc-16981-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa + TNFα Cell Lysate sc-2228 500 µg/200 µl $104
ERK 1 (m): 293T Lysate sc-126806 100 µg/200 µl $205
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Jurkat + PMA Cell Lysate sc-24718 500 µg/200 µl $104

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively (1–3). ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif (4,5). Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation (6). The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop (6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (4,5). Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1 (5). The ERK family has three additional members: ERK 3, ERK 5 and ERK 6 (7,8).
p-ERK 1/2 Antibody (Thr 177)-R Data
Click on image to enlarge
sc-16981-R
p-ERK 1/2 (Thr 177)-R: sc-16981-R. Western blot analysis of ERK 1/2 phosphorylation in untreated (A), UV irradiated (B) and UV irradiated and lambda protein phosphatase treated (C) HeLa whole cell lysates.
sc-16981-R
Western blot analysis of ERK 1/2 phosphorylation in untreated (A, D), UV irradiated (B, E) and UV irradiated and lambda protein phosphatase treated (C, F) HeLa whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 177/Thr 160)-R: sc-16981-R (A, B, C) and ERK 2 (K-23): sc-153 (D, E, F).
sc-16981-R
Western blot analysis of ERK 1/2 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 177 )-R: sc-16981-R (A,B,C) and ERK 2 (K-23): sc-153 (D,E,F).
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