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c-Jun (H-79) Antibody: sc-1694

 |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to amino acids 1-79 mapping at the N-terminus of c-Jun p39 of human origin
  • recommended for detection of c-Jun p39 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-1694 X, 200 µg/0.1 ml
  • agarose conjugate for IP studies, sc-1694 AC, 500 µg/0.25 ml agarose
  • fluorescein (sc-1694 FITC) and rhodamine (sc-1694 TRITC) conjugates for immunofluorescence, 200 µg/ml
  • phycoerythrin conjugate for intracellular FCM, sc-1694 PE, 100 tests
  • Alexa Fluor® 405 (sc-1694 AF405), Alexa Fluor® 488 (sc-1694 AF488) and Alexa Fluor® 647 (sc-1694 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
 
Additional c-Jun Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human JUN 3725 1p32.1 NM_002228 P05412
165160
Mouse Jun 16476 4 C5 P05627
N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun (H-79) sc-1694 200 µg/ml $279
c-Jun (H-79) AC sc-1694 AC 500µg/ml, 25%ag $366
c-Jun (H-79) Alexa Fluor® 405 sc-1694 AF405 100 µg/2 ml $314
c-Jun (H-79) Alexa Fluor® 488 sc-1694 AF488 100 µg/2 ml $314
c-Jun (H-79) Alexa Fluor® 647 sc-1694 AF647 100 µg/2 ml $314
c-Jun (H-79) FITC sc-1694 FITC 200 µg/ml $282
c-Jun (H-79) PE sc-1694 PE 100 tests in 2ml $303
c-Jun (H-79) TRITC sc-1694 TRITC 200 µg/ml $282
c-Jun (H-79) X sc-1694 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun siRNA (h) sc-29223 10 µM $258
c-Jun siRNA (m) sc-29224 10 µM $258
c-Jun siRNA (h2) sc-44201 10 µM $258
c-Jun (h)-PR sc-29223-PR 10 µM $23
c-Jun (m)-PR sc-29224-PR 10 µM $23
c-Jun (h2)-PR sc-44201-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun shRNA Plasmid (h) sc-29223-SH 20 µg $520
c-Jun shRNA Plasmid (m) sc-29224-SH 20 µg $520
c-Jun shRNA Plasmid (h2) sc-44201-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
c-Jun shRNA (h) Lentiviral Particles sc-29223-V 200 µl $625
c-Jun shRNA (m) Lentiviral Particles sc-29224-V 200 µl $625
c-Jun shRNA (h2) Lentiviral Particles sc-44201-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 + PMA nuclear extract sc-2125 1000 µg $143
Jurkat nuclear extract sc-2132 1000 µg $143
c-Jun (h): CHO Lysate sc-110019 100µg/200µl $205
c-Jun (h): 293 Lysate sc-110759 100µg/200µl $205
c-Jun (m): 293T Lysate sc-125069 100µg/200µl $205
c-Jun (m2): 293T Lysate sc-125070 100µg/200µl $205
c-Jun (m3): 293T Lysate sc-125071 100µg/200µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104

c-Jun Background Information
Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).

c-Jun (H-79) Product Citations
See how others have used c-Jun (H-79): sc-1694 antibody and or c-Jun (H-79) antibody conjugates.


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c-Jun (H-79)
Click on image to enlarge
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun p39 expression in untreated (A) and phorbol ester-induced (B) NIH/3T3 nuclear extracts.
c-Jun (H-79): sc-1694. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human prostate tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing nuclear localization.
Western blot analysis of non-phosphorylated human recombinant c-Jun fusion protein (A,C,E) phosphorylated by human recombinant JNK1 (B,D,F). Antibodies tested include c-Jun (H-79): sc-1694 (A,B), p-c-Jun (Ser 73)-R: sc-7981-R (C,D) and p-c-Jun (Ser 63/73)-R: sc-16312-R (E,F).
c-Jun (H-79) PE: sc-1694 PE. Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and PMA-induced (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: sc-3871.
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun expression in non-transfected: sc-110760 (A) and human c-Jun transfected: sc-110759 (B) 293 whole cell lysates.
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates.
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun expression in non-transfected 293T: sc-117752 (A), mouse c-Jun transfected 293T: sc-125070 (B) and NIH/3T3 (C) whole cell lysates.
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun expression in non-transfected 293T: sc-117752 (A), mouse c-Jun transfected 293T: sc-125071 (B) and NIH/3T3 (C) whole cell lysates.
c-Jun (H-79): sc-1694. Western blot analysis of c-Jun expression in non-transfected CHO: sc-117750 (A), human c-Jun transfected CHO: sc-110019 (B) and NIH/3T3 (C) whole cell lysates.
Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Jun transfected: sc-125069 (B,E) and lambda protein phosphatase (sc-200312A) treated human c-Jun transfected: sc-125069 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Jun (Ser 63/73)-R: sc-16312-R (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
Western blot analysis of c-Jun phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-c-Jun (Ser 63/73)-R: sc-16312-R (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Jun transfected: sc-125069 (B,E) and lambda protein phosphatase (sc-200312A) treated human c-Jun transfected: sc-125069 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Jun (KM-1): sc-822 (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
Western blot analysis of c-Jun phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-c-Jun (KM-1): sc-822 (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Jun transfected: sc-125069 (B,E) and lambda protein phosphatase (sc-200312A) treated human c-Jun transfected: sc-125069 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Jun (Ser 73)-R: sc-7981-R (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
Western blot analysis of c-Jun phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-c-Jun (Ser 73)-R: sc-7981-R (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
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