p-TFIIF RAP 74 Antibody : sc-16793-R

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In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression (1–3). The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site (2–5). TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit (6,7), is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB (6,7). In addition, TFIIF stimulates transcription elongation by RNA polymerase II (5).