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p-SGK (Thr 256) Antibody: sc-16744 |
| Datasheet |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-16744-R
- recommended for detection of Thr 256 phosphorylated SGK of mouse, rat and human origin by WB, IP, IF and ELISA
- blocking peptide, sc-16744 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
SGK |
6446 |
6q23.2 |
NM_005627 |
O00141
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602958 |
| Mouse |
Sgk1 |
20393 |
10 A3 |
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Q9WVC6
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N/A |
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SGK Background Information
Serum- and glucocorticoid-regulated kinase (SGK), a serine/threonine protein kinase, is transcriptionally regulated by serum, glucorticoids and mineralocorticoids. SGK regulates the control of extracellular fluid volume, blood pressure and sodium homeostasis and is also a component of the p38 MAPK-mediated response to hyperosmotic stress. SGK is a downstream target of phosphoinositide 3-kinase (PI 3-kinase)-stimulated growth factor signaling, and 3-phosphoinositide-dependent protein kinase 1 (PDK1) is capable of phosphorylating the activation-loop of SGK at Thr 256. Thr 256 and Ser 422 are the putative phosphorylation sites of SGK. Mutations at those putative phosphorylation sites inhibit SGK activation. For example, the Ser 422 to Ala mutant, lacking a PDK-2 phosphorylation site, is inactive and resistant to activation by Insulin. Thus, in addition to regulation at the level of gene expression, the enzymatic activity of SGK is regulated by multiple protein kinases, including PKA, PDK1, and PDK2.
| p-SGK (Thr 256) Product Citations |
See how others have used p-SGK (Thr 256): sc-16744 antibody and or p-SGK (Thr 256) antibody conjugates.
4 total citations Loading citations.
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p-SGK (Thr 256)
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Western blot analysis of inactive (A,C,E) and active (B,D,F) human recombinant SGK fusion protein. Antibodies tested include SGK (C-20): sc-15885 (A,B), p-SGK (Thr 256): sc-16744 (C,D) and p-SGK (Thr 256)-R: sc-16744-R (E,F). p-SGK (Ser 256)-R: sc-16744-R. Western blot analysis of SGK phosphorylation in untreated (A), insulin treated (B) and insulin and lambda protein phosphatase treated (C) NIH/3T3 whole cell lysates.
p-SGK (Thr 256)-R: sc-16744-R. Western blot analysis of SGK phosphorylation in PC-12 whole cell lysate.
Western blot analysis of SGK phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) HeLa whole cell lysates. Antibodies tested include p-SGK (Thr 256)-R: sc-16744-R (A,B) and SGK (C-20): sc-15885 (C,D). Western blot analysis of SGK phosphorylation in untreated (A,C) and lambda protein phosphatase ( sc-200312A) treated (B,D) Hep G2 whole cell lysates. Antibodies tested include p-SGK (Thr 256)-R: sc-16744-R (A,B) and SGK (C-20): sc-15885 (C,D). |
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