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p-Adducin α Antibody (Thr 480)-R: sc-16737-R

 |  Datasheet

(Based on data analysis)
  • p-Adducin α (Thr 480)-R Antibody is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to phosphorylated Thr 480 of Adducin α of rat origin
  • recommended for detection of Thr 480 phosphorylated Adducin α of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and bovine
  • blocking peptide, sc-16737 P
 

See additional Adducin Antibodies including Adducin, Adducin alpha, Adducin beta and Adducin gamma.

Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IF   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-Adducin α (Thr 480) P sc-16737 P
(peptide)
 100 µg/0.5 ml $61
p-Adducin α (Thr 480)-R Antibody sc-16737-R 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Adducin α siRNA (h) sc-43253 10 µM $258
Adducin α siRNA (m) sc-43254 10 µM $258
Adducin α (h)-PR sc-43253-PR 10 µM, 20 µl $23
Adducin α (m)-PR sc-43254-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Adducin α shRNA Plasmid (h) sc-43253-SH 20 µg $520
Adducin α shRNA Plasmid (m) sc-43254-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Adducin α shRNA (h) Lentiviral Particles sc-43253-V 200 µl $625
Adducin α shRNA (m) Lentiviral Particles sc-43254-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Adducin α (m): 293T Lysate sc-118250 100 µg/200 µl $205
Adducin β (h2): 293T Lysate sc-117077 100 µg/200 µl $205
Adducin γ(m): 293T Lysate sc-118249 100 µg/200 µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
K-562 + PMA Cell Lysate sc-2280 500 µg/200 µl $104

Adducins are a family of cytoskeleton proteins encoded by three genes (a, b and g). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. Adducins promote association of spectrin with actin and cap the fast growing end of actin filaments. Adducins contain an N-terminal core, neck and C-terminal tail domains, are substrates for protein kinase A (PKA) and C (PKC), and bind to Ca2+/calmodulin. The major phosphorylation sites common to the Adducins are Ser 726 and Ser 713 in the C-terminal MARCKS-related domains of Adducin a and Adducin b, and they are phosphorylated by PKA and PKC, respectively. In addition, PKA phosphorylates Adducin a at Ser 408, 436 and 481. Calmodulin-binding is inhibited by phosphorylation of Adducin b that, in turn, inhibits the rate of phospho-rylation of Adducin b, but not Adducin a. Rho-kinase can phosphorylate Adducin a at Thr 445 and Thr 480 downstream of Rho in vivo. The phosphorylation of Adducin by Rho-kinase plays an important role in the regulation of membrane ruffling and cell motility. In addition, phosphorylation at Ser 726 of Adducin a is required for cleavage by caspase-3 .