p-Adenovirus-2 E1A (Ser 89)-R Antibody: sc-16712-R

Adenovirus-2 E1A Background Information
The early region (E1) of the adenovirus genome, which is responsible for transforming activity, is localized within the leftmost 11% of the viral genome and consists of two transcriptional units, E1A and E1B (1). Human adenovirus early region 1A (E1A) products act as transcriptional regulators and function by altering DNA synthesis and inducing cell transformation (2). Adenovirus E1A proteins are phosphorylated nuclear oncoproteins that derive transforming activity largely through interactions with cellular proteins, including the tumor suppressor p105/Rb-1, cyclin A, a regulatory subunit associated with p34cdc2, and the related protein kinase p33cdk2. Both Ser-89 and Ser-219 are the major E1A phosphorylation sites that are phosphorylated in vitro by p34cdc2 (2–4). Phosphorylation of Ser-89 does not affect E1A-mediated repression of the simian virus 40 enhancer or trans-activation of E3 promoter significantly, but it has an effect on transformation of primary rat kidney cells (3,5).