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p-HSP 27 Antibody (D-3): sc-166694

 |  Datasheet

(Based on data analysis)

  • p-HSP 27 Antibody (D-3) is a mouse monoclonal IgG2b provided at 200 µg/ml
  • specific for an epitope containing phosphorylated Ser 82 of HSP 27 of human origin
  • recommended for detection of Ser 82 phosphorylated HSP 27 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
 

See additional HSP Antibodies including HSP, HSP 78, HSP 10, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60, HSP 65, HSC 70, HSP 70, HSP 75, HSP 90 alpha, HSP 90 beta, Hsp90, Hsp104 and HSP 105.

Also see HSP 27 Inhibitors for functional analysis of cellular responses to HSP 27.


Ordering InformationGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-HSP 27 Antibody (D-3) sc-166694 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HSP 27 siRNA (h) sc-29350 10 µM $258
HSP 27 siRNA (m) sc-35598 10 µM $258
HSP 27 siRNA (h2) sc-44264 10 µM $258
HSP 27 (h)-PR sc-29350-PR 10 µM, 20 µl $23
HSP 27 (m)-PR sc-35598-PR 10 µM, 20 µl $23
HSP 27 (h2)-PR sc-44264-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HSP 27 shRNA Plasmid (h) sc-29350-SH 20 µg $520
HSP 27 shRNA Plasmid (m) sc-35598-SH 20 µg $520
HSP 27 shRNA Plasmid (h2) sc-44264-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HSP 27 shRNA (h) Lentiviral Particles sc-29350-V 200 µl $625
HSP 27 shRNA (m) Lentiviral Particles sc-35598-V 200 µl $625
HSP 27 shRNA (h2) Lentiviral Particles sc-44264-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa + heat shock Cell Lysate sc-2272 500 µg/200 µl $104
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
ECV304 Cell Lysate sc-2269 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HSP 27 (h): 293T Lysate sc-174710 100 µg/200 µl $205
HSP 27 (h2): 293T Lysate sc-174711 100 µg/200 µl $205
HSP 27 (h4): 293T Lysate sc-174723 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human HSPB1 3315 7q11.23 P04792
 608634
Mouse Hspb1 15507 5 G2 P14602
 N/A
 

HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.

p-HSP 27 Antibody (D-3) Data
Click on image to enlarge
sc-166694
Western blot analysis of HSP 27 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-HSP 27 (D-3): sc-166694 (A,B,C) and HSP 27 (F-4): sc-13132 (D,E,F).
sc-166694
p-HSP 27 (D-3): sc-166694. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.
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