goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-16667-R
epitope corresponding to a short amino acid sequence containing phosphorylated Ser 57 of NF-L of human origin
recommended for detection of Ser 57 phosphorylated NF-L of mouse, rat and human origin of WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
NF-L Background Information Neurofilament-L (for neurofilament light polypeptide, or NF-L) is a member of the intermediate filament family and a major component of neuronal cytoskeletons (1). Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma (2–5). In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments (6). Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease (7,8). Calcium/calmodulin-dependent protein kinase II (CamKII) phosphorylates NF-L at Ser 57 under induced long term potentiation. This phosphorylation event suggests a relationship between neuronal signal transduction and phosphorylation at
Ser 57 in vivo (9).
