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p-FAK Antibody (Tyr 577): sc-16665

 |  Datasheet

(Based on data analysis)

  • p-FAK Antibody (Tyr 577) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-16665-R
  • epitope corresponding to a short amino acid sequence containing phosphorylated Tyr 577 of focal adhesion kinase (FAK) of human origin
  • recommended for detection of Tyr 577 phosphorylated FAK of mouse, rat and human origin by WB, IP and IF; also reactive with additional species, including equine, canine, bovine and avian
  • blocking peptide, sc-16665 P
  • See 2 citations for p-FAK Antibody (Tyr 577)
 

See additional FAK Antibodies.

Also see FAK Inhibitors or FAK Activators for functional analysis of cellular responses to FAK.


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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-FAK Antibody (Tyr 577) sc-16665 200 µg/ml $279
p-FAK (Tyr 577) P sc-16665 P
(peptide)
 100 µg/0.5 ml $61
p-FAK Antibody (Tyr 577)-R sc-16665-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
FAK siRNA (h) sc-29310 10 µM $258
FAK siRNA (m) sc-35353 10 µM $258
FAK (h)-PR sc-29310-PR 10 µM, 20 µl $23
FAK (m)-PR sc-35353-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
FAK shRNA Plasmid (h) sc-29310-SH 20 µg $520
FAK shRNA Plasmid (m) sc-35353-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
FAK shRNA (h) Lentiviral Particles sc-29310-V 200 µl $625
FAK shRNA (m) Lentiviral Particles sc-35353-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 + anisomycin Cell Lysate sc-2247 500 µg/200 µl $104
FAK (h): 293T Lysate sc-114600 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human PTK2 5747 8q24.3 NM_005607, NM_153831 Q05397
 600758
Mouse Ptk2 14083 15 D3 P34152
 N/A
 

Activation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phospho-rylation of FAK Tyr 397 decreases during serum starvation, contact inhibition and cell cycle arrest, all conditions under which activating FAK Tyr 407 phosphorylation increases.

p-FAK Antibody (Tyr 577) Product Citations
See how others have used p-FAK Antibody (Tyr 577) and or p-FAK Antibody (Tyr 577) conjugates.


2 total citations
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p-FAK Antibody (Tyr 577) Data
Click on image to enlarge
sc-16665
p-FAK (Tyr 577): sc-16665. Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A) and human FAK transfected: sc-114600 (B) 293T whole cell lysates.
sc-16665
p-FAK (Tyr 577)-R: sc-16665-R. Western blot analysis of FAK phosphorylation in HeLa whole cell lysate.
sc-16665
Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A, D), untreated human FAK transfected: sc-114600 (B, E) and lambda protein phosphatase (sc-200312A) treated human FAK transfected: sc-114600 (C, F) 293T whole cell lysates. Antibodies tested include p-FAK (Tyr 577)-R: sc-16665-R (A, B, C) and FAK (H-1): sc-1688 (D, E, F).
sc-16665
Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A,D), untreated human FAK transfected: sc-114600 (B,E) and lambda protein phosphatase treated human FAK transfected: sc-114600 (C,F) 293T whole cell lysates. Antibodies tested include p-FAK (Tyr 577)-R: sc-16665-R (A,B,C) and FAK (C-903): sc-932 (D,E,F).
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