goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-16664-R
epitope corresponding to a short amino acid sequence containing phosphorylated Tyr 407 of focal adhesion kinase (FAK) of human origin
recommended for detection of Tyr 407 phosphorylated FAK of mouse, rat and human origin by WB and IF; also reactive with additional species, including equine, canine, bovine and avian
FAK Background Information Activation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phospho-rylation of FAK Tyr 397 decreases during serum starvation, contact inhibition and cell cycle arrest, all conditions under which activating FAK Tyr 407 phosphorylation increases.
