ESET Antibody (A-1): sc-166621

ESET Antibody (A-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects ESET protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ESET antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ERG-associated protein with SET domain (ESET), also known as Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, is a crucial nuclear protein that belongs to the histone-lysine methyltransferase family and the Suvar3-9 subfamily. This highly conserved protein, consisting of 150 amino acids, plays a significant role in chromatin structure modulation, which is essential for regulating gene expression and maintaining cellular identity. ESET is specifically localized to nonpericentromeric regions of chromatin and is excluded from cell nucleoli and areas of condensed chromatin, highlighting its importance in epigenetic regulation. The gene encoding ESET, SETDB1, is located on chromosome 1q21, and mutations in this gene can disrupt methyltransferase activity, leading to altered gene expression patterns. ESET functions by methylating Lys-9 of histone H3, a modification that serves as a signal for epigenetic transcriptional repression by recruiting heterochromatin protein 1 (HP1) to methylated histones. ESET is widely expressed, with highest levels found in testis, underscoring its potential role in reproductive biology and development.

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ESET Antibody (A-1) References:

1. Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids.  |  Harte, PJ., et al. 1999. Cytogenet Cell Genet. 84: 83-6. PMID: 10343109
2. Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor.  |  Yang, L., et al. 2002. Oncogene. 21: 148-52. PMID: 11791185
3. mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.  |  Wang, H., et al. 2003. Mol Cell. 12: 475-87. PMID: 14536086
4. Histone H3-K9 methyltransferase ESET is essential for early development.  |  Dodge, JE., et al. 2004. Mol Cell Biol. 24: 2478-86. PMID: 14993285
5. Histone hypomethylation is an indicator of epigenetic plasticity in quiescent lymphocytes.  |  Baxter, J., et al. 2004. EMBO J. 23: 4462-72. PMID: 15510223
6. ESET/SETDB1 gene expression and histone H3 (K9) trimethylation in Huntington's disease.  |  Ryu, H., et al. 2006. Proc Natl Acad Sci U S A. 103: 19176-81. PMID: 17142323
7. ESET histone methyltransferase regulates osteoblastic differentiation of mesenchymal stem cells during postnatal bone development.  |  Lawson, KA., et al. 2013. FEBS Lett. 587: 3961-7. PMID: 24188826
8. ESET methylates UBF at K232/254 and regulates nucleolar heterochromatin plasticity and rDNA transcription.  |  Hwang, YJ., et al. 2014. Nucleic Acids Res. 42: 1628-43. PMID: 24234436
9. The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice.  |  An, J., et al. 2014. Cell Death Dis. 5: e1196. PMID: 24763053
10. A Histone Methyltransferase ESET Is Critical for T Cell Development.  |  Takikita, S., et al. 2016. J Immunol. 197: 2269-79. PMID: 27511731
11. Epigenetic inhibition of lncRNA GMDS-AS1 by methyltransferase ESET promoted cell viability and metastasis of hepatocellular carcinoma.  |  Huang, J., et al. 2023. Clin Transl Oncol. 25: 1793-1804. PMID: 36737533
12. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1.  |  Nomura, N., et al. 1994. DNA Res. 1: 223-9. PMID: 7584044

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ESET Antibody (A-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ESET Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ESET Antibody (A-1) Specifications

Alternate Product Name: SETDB1

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1195-1228

Epitope Target: ESET

Epitope Species: human

Product Reactivity/Detection: ESET

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ESET Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent