BTF3a/b Antibody (A-5): sc-166093
- BTF3a/b Antibody (A-5) is a mouse monoclonal IgG1 κ BTF3a/b antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-206 representing full length BTF3a of human origin
- BTF3a/b Antibody (A-5) is recommended for detection of BTF3a/b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-BTF3a/b Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BTF3a/b (A-5): sc-166093.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BTF3a/b Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with BTF3a/b Antibody (A-5) (see ordering information below).
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BTF3a/b Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects BTF3a/b of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BTF3a/b (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BTF3 plays a crucial role in the initiation of gene transcription, which is essential for cellular function and regulation. BTF3 is part of a multiprotein complex that assembles on proximal promoter elements, such as the TATA box, to facilitate RNA polymerase II and other transcription factors binding. BTF3 gene encodes two isoforms, BTF3a and BTF3b, produced through alternative splicing; while both isoforms can bind RNA polymerase II, only BTF3a induces transcription due to a critical N-terminal region absent in BTF3b. This structural difference highlights BTF3a′s importance in transcriptional regulation, as BTF3a not only interacts with RNA polymerase II but also associates with protein kinase CK2, which phosphorylates BTF3a and enables efficient transcription of tRNA and 55 rRNA genes by RNA polymerase III. BTF3 proteins′ ubiquitous expression underscores their fundamental role in gene expression across various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BTF3a/b Antibody (A-5) References:
- BTF3 is a potential new substrate of protein kinase CK2. | Grein, S. and Pyerin, W. 1999. Mol Cell Biochem. 191: 121-8. PMID: 10094400
- A review of progress towards elucidating the role of protein kinase CK2 in polymerase III transcription: regulation of the TATA binding protein. | Ghavidel, A., et al. 1999. Mol Cell Biochem. 191: 143-8. PMID: 10094403
- Genomic structure of the putative BTF3 transcription factor. | Kanno, M., et al. 1992. Gene. 117: 219-28. PMID: 1386332
- Class II (B) general transcription factor (TFIIB) that binds to the template-committed preinitiation complex is different from general transcription factor BTF3. | Moncollin, V., et al. 1992. Proc Natl Acad Sci U S A. 89: 397-401. PMID: 1729710
- Sequencing and expression of complementary DNA for the general transcription factor BTF3. | Zheng, XM., et al. 1990. Nature. 344: 556-9. PMID: 2320128
- A general transcription factor forms a stable complex with RNA polymerase B (II). | Zheng, XM., et al. 1987. Cell. 50: 361-8. PMID: 3607874
- A spectrum of mechanisms for the assembly of the RNA polymerase II transcription preinitiation complex. | George, CP., et al. 1995. Mol Cell Biol. 15: 1049-59. PMID: 7823920
- Multiple sets of basal factors initiate transcription by RNA polymerase II. | Parvin, JD., et al. 1994. J Biol Chem. 269: 18414-21. PMID: 8034589
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTF3a/b Antibody (A-5) | sc-166093 | 200 µg/ml | $316.00 | |||
BTF3a/b Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-528905 | 200 µg Ab; 10 µg BP | $354.00 | |||
BTF3a/b Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-521507 | 200 µg Ab, 40 µg BP | $354.00 | |||
BTF3a/b Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-543201 | 200 µg Ab; 20 µg BP | $354.00 | |||
BTF3a/b Antibody (A-5) AC | sc-166093 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BTF3a/b Antibody (A-5) HRP | sc-166093 HRP | 200 µg/ml | $316.00 | |||
BTF3a/b Antibody (A-5) FITC | sc-166093 FITC | 200 µg/ml | $330.00 | |||
BTF3a/b Antibody (A-5) PE | sc-166093 PE | 200 µg/ml | $343.00 | |||
BTF3a/b Antibody (A-5) Alexa Fluor® 488 | sc-166093 AF488 | 200 µg/ml | $357.00 | |||
BTF3a/b Antibody (A-5) Alexa Fluor® 546 | sc-166093 AF546 | 200 µg/ml | $357.00 | |||
BTF3a/b Antibody (A-5) Alexa Fluor® 594 | sc-166093 AF594 | 200 µg/ml | $357.00 | |||
BTF3a/b Antibody (A-5) Alexa Fluor® 647 | sc-166093 AF647 | 200 µg/ml | $357.00 | |||
BTF3a/b Antibody (A-5) Alexa Fluor® 680 | sc-166093 AF680 | 200 µg/ml | $357.00 | |||
BTF3a/b Antibody (A-5) Alexa Fluor® 790 | sc-166093 AF790 | 200 µg/ml | $357.00 |