goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-16598-R
epitope corresponding to a short amino acid sequence containing phosphorylated Tyr 620 of MAG (myelin-associated glycoprotein) of human origin
recommended for detection of Tyr 620 phosphorylated MAG of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and bovine
MAG Background Information Myelin-associated glycoprotein (MAG) is a nervous system specific, cell-surface adhesion protein that is involved in linking myelinating glial cells to neuronal axons. MAG contains a sialic acid binding site and five IgG-like domains, thus identifying MAG as a member of a subgroup of the immunoglobulin superfamily. Like myelin, MAG inhibits axonal outgrowth and contributes to the inhibitory properties of myelin. Growth inhibition by MAG can be blocked when cerebellar neurons are pre-incubated with the neurotrophins BDNF or GDNF. This effect may result from neurotrophin pirming, which elevates cAMP and activates PKA. MAG is phosphorylated at Tyr 620 in developing brain and subsequently associates with the SH2 domain of PLCg. MAG also binds Fyn kinase, and the binding of phosphorylated MAG to both Fyn kinase and PLCg suggests MAG serves as a docking protein for different signaling molecules.
