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pan 14-3-3 (H-8) Antibody: sc-1657 |
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- mouse monoclonal IgG2b; 200 µg/ml
- specific for an epitope mapping between amino acids 1-30 at the N-terminus of pan 14-3-3 of human origin
- recommended for detection of members of the 14-3-3 protein family of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-1657 P
- fluorescein (sc-1657 FITC) and rhodamine (sc-1657 TRITC) conjugates for immunofluorescence, 200 µg/ml
- HRP conjugate, sc-1657 HRP, 200 µg/ml
- phycoerythrin conjugate for intracellular FCM, sc-1657 PE, 100 tests
- Alexa Fluor® 405 (sc-1657 AF405), Alexa Fluor® 488 (sc-1657 AF488) and Alexa Fluor® 647 (sc-1647 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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pan 14-3-3 Background Information
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
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pan 14-3-3 (H-8)
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pan 14-3-3 (H-8): sc-1657. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
pan 14-3-3 (H-8): sc-1657. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue (A). Note cytoplasmic and membrane staining of glandular epithelia. Immunofluorescence staining of methanol-fixed HeLa cells. Note cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain (B).
Western blot analysis of 14-3-3 expression in HeLa whole cell lysates (A,B). Antibodies tested include pan 14-3-3 (H-8): sc-1657 (A) and pan 14-3-3 (K-19): sc-629 (B). pan 14-3-3 (H-8) PE: sc-1657 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-2868. pan 14-3-3 (H-8) Alexa Fluor 488: sc-1657 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-3892. pan 14-3-3 (H-8): sc-1657. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor® 488 (B) staining.
pan 14-3-3 (H-8): sc-1657. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
pan 14-3-3 (H-8): sc-1657. Western blot analysis of 14-3-3 ζ expression in non-transfected 293: sc-110760 (A), human 14-3-3 ζ transfected 293: sc-175740 (B) and HeLa (C) whole cell lysates. |
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