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- mouse monoclonal IgG1; 200 µg/ml
- raised against amino acids 1-197 representing full length p27 of mouse origin
- recommended for detection of p27 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
- agarose conjugate for IP studies, sc-1641 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-1641 FITC) and rhodamine (sc-1641 TRITC) conjugates for immunofluorescence, 200 µg/ml
- phycoerythrin conjugate for intracellular FCM, sc-1641 PE, 100 tests
- Alexa Fluor® 405 (sc-1641 AF405), Alexa Fluor® 488 (sc-1641 AF488) and Alexa Fluor® 647 (sc-1641 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
CDKN1B |
1027 |
12p13.1 |
NM_004064 |
P46527
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610755 |
| Mouse |
Cdkn1b |
12576 |
6 G1 |
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P46414
|
N/A |
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p27 Background Information
Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGF∫-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2.
| p27 (F-8) Product Citations |
See how others have used p27 (F-8): sc-1641 antibody and or p27 (F-8) antibody conjugates.
181 total citations Loading citations.
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p27 (F-8)
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p27 (F-8): sc-1641. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of p27.
p27 (F-8): sc-1641. Western blot analysis of p27 expression in MM-142 (A) and KNRK (B) whole cell lysates.
Western blot analysis of mouse recombinant p27 (A,C,E) and mouse recombinant p27 phosphorylated by rat recombinant ERK2 (B,D,F). Antibodies tested include p27 (F-8): sc-1641 (A,B), p-p27 (Ser 10)-R: sc-12939-R (C,D) and p-p27 (Thr 187)-R: sc-16324-R (E,F). p27 (F-8): sc-1641. Western blot analysis of p27 expression in non-transfected: sc-110760 (A) and human p27 transfected: sc-110470 (B) 293 whole cell lysates. p27 (F-8): sc-1641. Western blot analysis of p27 expression in non-transfected: sc-117752 (A) and mouse p27 transfected: sc-122312 (B) 293T whole cell lysates. p27 (F-8): sc-1641. Western blot analysis of p27 expression in Jurkat whole cell lysate.
p27 (F-8): sc-1641. Western blot analysis of p27 expression in untreated (A) and Dibutyryl-cAMP ( sc-201567) treated (B) SH-SY5Y whole cell lysates. Note down regulation of p27 expression in lane B. p27 (F-8) PE: sc-1641 PE. Intracellular FCM analysis of fixed and permeabilized KNRK cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2866. |
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