p-Vav2 (Tyr 172)-R Antibody: sc-16409-R

Vav2 Background Information
Vav proteins are guanine nucleotide exchange factors for Rho family GTPases which activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. Vav proteins contain several protein binding domains which can link cell surface receptors to downstream signaling proteins. Vav3 is a Ros receptor protein tyrosine kinase (RPTK) interacting protein and has a broad tissue expression profile that is distinct from those of Vav and Vav2. Vav3 mediates RPTK signaling and regulates GTPase activity, its native and mutant forms are able to modulate cell morphology, and it has the potential to induce cell transformation. For example, Vav3 induces marked membrane ruffles and microspikes in NIH/3T3 cells. Vav works as a GDP/GTP exchange factor for Rac GTPases, thereby facilitating the transition of these proteins from the inactive (GDP-bound) into the active (GTP-bound) state. The stimulation of Vav exchange activity during cell signaling is mediated by tyrosine phosphorylation. The residue, Tyrosine 174, is phospho-rylated following the stimulation of mitogenic and antigenic receptors. This phosphorylation event is conserved in Vav2 and Vav3, the other two members of the Vav family.