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home » Kinases and Phosphatases » Akt Antibody / Akt Antibodies » Akt1 (C-20): sc-1618

Akt1 (C-20) Antibody: sc-1618

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-1618-R
  • epitope mapping at the C-terminus of Akt1 of human origin
  • recommended for detection of Akt1 and, to a lesser extent, Akt2 and Akt3 of mouse, rat, human and Xenopus origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • blocking peptide, sc-1618 P
  • agarose conjugate for IP studies, sc-1618 AC, 500 µg/0.25 ml agarose
  • phycoerythrin (sc-1618 PE) conjugate for intracellular FCM, 100 tests
 
Additional Akt Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   FCM  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human AKT1 207 14q32.33 NM_001014431, NM_001014432, NM_005163 P31749
 604370
Mouse Akt1 11651 12 F1 P31750
 N/A
Mouse Akt2 11652 7 A3 Q60823
 N/A
Mouse Akt3 23797 1 H4 Q9WUA6
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Akt1 (C-20) sc-1618 200 µg/ml $279
Akt1 (C-20) AC sc-1618 AC 500µg/ml, 25%ag $366
Akt1 (C-20) P sc-1618 P
(peptide)
 100 µg/0.5 ml $61
Akt1 (C-20) PE sc-1618 PE 100 tests in 2ml $303
Akt1 (C-20)-R sc-1618-R 200 µg/ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
MIA PaCa-2 Cell Lysate sc-2285 500 µg/200 µl $104
ZR-75-1 Cell Lysate sc-2241 500 µg/200 µl $104
BT-20 Cell Lysate sc-2223 500 µg/200 µl $104

Akt1 Background Information
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB∫ or RacPK-∫) and Akt 3 (also designated PKB© or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Akt1 (C-20) Product Citations
See how others have used Akt1 (C-20): sc-1618 antibody and or Akt1 (C-20) antibody conjugates.


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Akt1 (C-20)
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sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in MCF7 (A), KNRK (B) and NIH/3T3 (C) whole cell lysates.
sc-1618
Western blot analysis of Akt1 phosphorylation in A-431 cells treated with EGF. Blots were probed with Akt1 (C-20): sc-1618 (A), p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate unphosphorylated peptide (B) and p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate phosphorylated peptide (C).
sc-1618
Akt1 (C-20)-R: sc-1618-R. Western blot analysis of Akt1 expression in KNRK (A), NIH/3T3 (B), HeLa (C), ZR-75-1 (D) and BT-20 (E) whole cell lysates.
sc-1618
Akt1 (C-20): sc-1618. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt2 expression in non-transfected 293T: sc-117752 (A), human Akt2 transfected 293T: sc-116831 (B) and A-431 (C) whole cell lysates.
sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt2 expression in non-transfected 293T: sc-117752 (A), mouse Akt2 transfected 293T: sc-126407 (B) and KNRK (C) whole cell lysates.
sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in IMR-32 whole cell lysate.
sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in IMR-32 whole cell lysate.
sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in non-transfected: sc-117752 (A) and human Akt1 transfected: sc-176810 (B) 293T whole cell lysates.
sc-1618
Akt1 (C-20)-R: sc-1618-R. Western blot analysis of Akt1 expression in non-transfected: sc-117752 (A) and human Akt1 transfected: sc-176810 (B) 293T whole cell lysates.
sc-1618
Akt1 (C-20): sc-1618. Western blot analysis of Akt1 expression in non-transfected: sc-117752 (A) and human Akt1 transfected: sc-158248 (B) 293T whole cell lysates.
sc-1618
Western blot analysis of Akt1 phosphorylation in untreated (A,D), serum starved and insulin treated (B,E) and serum starved, insulin treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Akt1 (11E6): sc-81433 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
sc-1618
Western blot analysis of Akt1 phosphorylation in untreated (A,D), serum starved and insulin treated (B,E) and serum starved, insulin treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Akt1/2/3 (Ser 473): sc-33437 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
sc-1618
Western blot analysis of Akt1 phosphorylation in untreated (A,D), mouse LIF (sc-4989) treated (B,E) and LIF and lambda protein phosphatase (sc-200312A) treated (C,F) 3T3-L1 whole cell lysates. Antibodies tested include p-Akt1 (11E6): sc-81433 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
sc-1618
Akt1 (C-20) PE: sc-1618 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992.
sc-1618
Western blot analysis of Akt1 phosphorylation in untreated (A,D), mouse LIF treated (B,E), and mouse LIF and lambda protein phosphatase (sc-200312A) treated (C,F) 3T3-L1 whole cell lysates. Antibodies tested include p-Akt1 (104A282): sc-52940 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
sc-1618
Western blot analysis of Akt1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Akt1 transfected: sc-158248 (B,E) and lambda protein phosphatase (sc-200312A) treated human Akt1 transfected: sc-158248 (C,F) 293T whole cell lysates. Antibodies tested include p-Akt1/2/3 (A-12): sc-271964 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
sc-1618
Western blot analysis of Akt1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Akt1 transfected: sc-158248 (B,E) and lambda protein phosphatase (sc-200312A) treated human Akt1 transfected: sc-158248 (C,F) 293T whole cell lysates. Antibodies tested include p-Akt1/2/3 (B-5): sc-271966 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
sc-1618
Western blot analysis of Akt1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Akt1 transfected: sc-158248 (B,E) and lambda protein phosphatase (sc-200312A) treated human Akt1 transfected: sc-158248 (C,F) 293T whole cell lysates. Antibodies tested include p-Akt1 (Thr 308): sc-135650 (A,B,C) and Akt1 (C-20): sc-1618 (D,E,F).
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