Actin Antibody (I-19) | Santa Cruz Biotech

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Actin Antibody (I-19): sc-1616	\| Datasheet

5 5 (Based on data analysis) 1

Actin Antibody (I-19) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-1616-R
epitope mapping at the C-terminus of Actin of human origin
recommended for detection of a broad range of Actin isoforms of mouse, rat, human, zebrafish, C. elegans, Drosophila, and Xenopus origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-1616 P
agarose conjugate for IP studies, sc-1616 AC, 500 µg/0.25 ml agarose
fluorescein (sc-1616 FITC) and phycoerythrin (sc-1616 PE) conjugates for intracellular FCM, 100 tests
biotin conjugate, sc-1616 B, 200 µg/ml
HRP conjugate, sc-1616 HRP, 200 µg/ml
rhodamine (sc-1616 TRITC), conjugate for IHC, 200 µg/1 ml.
Alexa Fluor® 405 (sc-1616 AF405), Alexa Fluor® 488 (sc-1616 AF488) and Alexa Fluor® 647 (sc-1616 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
See 1002 citations for Actin Antibody (I-19)

See additional Actin Antibodies including Actin, alpha-Actin, beta-Actin, gamma-Actin, kappa-Actin, Muscle Actin and Smooth Muscle Actin.

Also see Actin Inhibitors for functional analysis of cellular responses to Actin.

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WB IP IF FCM siRNA

Ordering Information

Product Name	Catalog #	Unit	Price
Actin Antibody (I-19)	sc-1616	200 µg/ml	$279
Actin Antibody (I-19) AC	sc-1616 AC	500 µg/ml, 25% ag	$366
Actin Antibody (I-19) Alexa Fluor^® 405	sc-1616 AF405	100 µg/2 ml	$314
Actin Antibody (I-19) Alexa Fluor^® 488	sc-1616 AF488	100 µg/2 ml	$314
Actin Antibody (I-19) Alexa Fluor^® 647	sc-1616 AF647	100 µg/2 ml	$314
Actin Antibody (I-19) B	sc-1616 B	200 µg/ml	$282
Actin Antibody (I-19) FITC	sc-1616 FITC	100 tests in 2 ml	$292
Actin Antibody (I-19) HRP	sc-1616 HRP	200 µg/ml	$279
Actin (I-19) P	sc-1616 P (peptide)	100 µg/0.5 ml	$61
Actin Antibody (I-19) PE	sc-1616 PE	100 tests in 2 ml	$303
Actin Antibody (I-19) TRITC	sc-1616 TRITC	200 µg/ml	$282
Actin Antibody (I-19)-R	sc-1616-R	200 µg/ml	$279

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
C32 Whole Cell Lysate	sc-2205	500 µg/200 µl	$104
BC₃H1 Cell Lysate	sc-2299	500 µg/200 µl	$104
Sol8 Cell Lysate	sc-2249	500 µg/200 µl	$104
KNRK Whole Cell Lysate	sc-2214	500 µg/200 µl	$104
A-431 Whole Cell Lysate	sc-2201	500 µg/200 µl	$104
NIH/3T3 Whole Cell Lysate	sc-2210	500 µg/200 µl	$104
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	ACTA1	58	1q42.13	NM_001100	P68133	255310
Human	ACTA2	59	10q23.31	NM_001613	P62736	102620
Human	ACTB	60	7p22.1	NM_001101	P60709	102630
Human	ACTC1	70	15q14	NM_005159	P68032	192600
Human	ACTG1	71	17q25.3	NM_001614	P63261	604717
Human	ACTG2	72	2p13.1	NM_001615	P63267	102545
Mouse	Acta1	11459	8 E2		P68134	N/A
Mouse	Actb	11461	5 G2		P60710	N/A
Mouse	Actc1	11464	2 E4		P68033	N/A
Mouse	Actg1	11465	11 E2		P63260	N/A
Mouse	Actg2	11468	6 C3		P63268	N/A
Mouse	Acta2	11475	19 C1		P62737	N/A
Mouse	Actbl2	238880	13 D2.2		Q8BFZ3	N/A

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. å-Actin expression is limited to various types of muscle, whereas -∫ and ©-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Actin Antibody (I-19) Product Citations

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Actin Antibody (I-19) Data

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Actin (I-19): sc-1616. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal fluorescein immunostaining of actin filaments. Note nuclear rhodamine immunostaining with PCNA (PC-10): sc-56.

Actin (I-19): sc-1616. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic staining.

Actin (I-19): sc-1616. Western blot analysis of Actin expression in C32 (A), BC₃H1 (B) and Sol8 (C) whole cell lysates.

Actin (I-19) Alexa Fluor 488: sc-1616 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: sc-45067.

Actin (I-19) Alexa Fluor 647: sc-1616 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: sc-45066.

Actin (I-19): sc-1616. Western blot analysis of Actin expression in A-10 whole cell lysate.

Actin (I-19): sc-1616. Western blot analysis of ACTA1 expression in non-transfected: sc-117752 (A) and mouse ACTA1 transfected: sc-118228 (B) 293T whole cell lysates.

Actin (I-19): sc-1616. Western blot analysis of ACTG2 expression in non-transfected: sc-117752 (A) and mouse ACTG2 transfected: sc-118229 (B) 293T whole cell lysates.

Actin (I-19): sc-1616. Western blot analysis of γ-Actin expression in non-transfected 293T: sc-117752 (A), human γ-Actin transfected 293T: sc-116428 (B) and HeLa (C) whole cell lysates.

Actin (I-19): sc-1616. Western blot analysis of ACTG1 expression in non-transfected 293T: sc-117752 (A), mouse ACTG1 transfected 293T: sc-124922 (B), HeLa (C) and A-431(D) whole cell lysates.

Actin (I-19): sc-1616. Western blot analysis of β-Actin expression in non-transfected 293T: sc-117752 (A), human β-Actin transfected 293T: sc-111134 (B) and C32 (C) whole cell lysates.

Actin (I-19): sc-1616. Western blot analysis of γ-Actin-2 expression in non-transfected 293T: sc-117752 (A), human γ-Actin-2 transfected 293T: sc-176378 (B) and C32 (C) whole cell lysates.

Actin (I-19) FITC: sc-1616 FITC. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal Goat IgG: sc-3988.

Actin (I-19) PE: sc-1616 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992.

Actin (I-19): sc-1616. Western blot analysis of Actin expression in C₃2 (A), BC3HI (B), Sol 8 (C) and L8 (D) whole cell lysates.

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Actin Antibody (I-19): sc-1616