Welcome to Santa Cruz Biotechnology!
SCBT Home
Home » Transcription Regulators » PARP Antibodies » PARP-1 Antibody (A-20): sc-1562

PARP-1 Antibody (A-20): sc-1562

 |  Datasheet

(Based on data analysis)

  • PARP-1 Antibody (A-20) is a goat polyclonal IgG provided at 200 µg/ml
  • epitope mapping at the N-terminus of PARP of mouse origin
  • recommended for detection of full-length PARP-1 and the N-terminal cleavage product of PARP-1 of mouse, rat and human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA
  • blocking peptide, sc-1562 P
  • See 20 citations for PARP-1 Antibody (A-20)
 

See additional PARP Antibodies including PARP, pADPr, PARP L, PARP S, PARP-1, PARP-2, PARP-3, PARP-4, PARP-6, PARP-7, PARP-8, PARP-9, PARP-10, PARP-11, PARP-12, PARP-14, PARP-15 and PARP-16.

Also see PARP-1 Inhibitors for functional analysis of cellular responses to PARP-1.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
PARP-1 Antibody (A-20) sc-1562 200 µg/ml $279
PARP-1 (A-20) P sc-1562 P
(peptide)
 100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
PARP-1 siRNA (h) sc-29437 10 µM $258
PARP-1 siRNA (m) sc-29438 10 µM $258
PARP-1 (h)-PR sc-29437-PR 10 µM, 20 µl $23
PARP-1 (m)-PR sc-29438-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
PARP-1 shRNA Plasmid (h) sc-29437-SH 20 µg $520
PARP-1 shRNA Plasmid (m) sc-29438-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
PARP-1 shRNA (h) Lentiviral Particles sc-29437-V 200 µl $625
PARP-1 shRNA (m) Lentiviral Particles sc-29438-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
CCRF-CEM nuclear extract sc-2146 250 µg/0.05 ml $143
HeLa nuclear extract sc-2120 250 µg/0.05 ml $143
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Ramos nuclear extract sc-2153 250 µg/0.05 ml $143
Jurkat nuclear extract sc-2132 250 µg/0.05 ml $143
Daudi Cell Lysate sc-2415 500 µg/200 µl $104
PARP-1 (h): 293T Lysate sc-114869 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human PARP1 142 1q42.12 NM_001618 P09874
 173870
Mouse Parp1 11545 1 H4 P11103
 N/A
 

Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity.

PARP-1 Antibody (A-20) Product Citations
See how others have used PARP-1 Antibody (A-20) and or PARP-1 Antibody (A-20) conjugates.


20 total citations
Loading citations.
 
PARP-1 Antibody (A-20) Data
Click on image to enlarge
sc-1562
Western blot analysis of PARP expression in Jurkat whole cell lysates (A,B). Antibodies tested include PARP-1 (N-20): sc-1561 (A) and PARP-1 (A-20): sc-1562 (B).
sc-1562
PARP-1 (A-20): sc-1562. Western blot analysis of PARP-1 expression in non-transfected: sc-117752 (A) and human PARP-1 transfected: sc-114869 (B) 293T whole cell lysates.
sc-1562
PARP-1 (A-20): sc-1562. Western blot analysis of PARP-1 expression in non-transfected: sc-117752 (A) and mouse PARP-1 transfected: sc-122382 (B) 293T whole cell lysates.
sc-1562
PARP-1 (A-20): sc-1562. Western blot analysis of PARP-1 expression in Jurkat (A), HeLa (B), K-562 (C) and NIH/3T3 (D) nuclear extracts.
sc-1562
PARP-1 (A-20): sc-1562. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear staining of cartilage cells.
Download
Datasheets
Get Adobe Acrobat Reader