epitope mapping at the N-terminus of PARP of human origin
recommended for detection of full-length PARP-1 and the N-terminal cleavage product of PARP-1 of mouse, rat and human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine and porcine
PARP-1 Background Information Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity.
PARP-1 (N-20) Product Citations
See how others have used PARP-1 (N-20): sc-1561 antibody and or PARP-1 (N-20) antibody conjugates.
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PARP-1 (N-20)
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Western blot analysis of PARP expression in Jurkat whole cell lysates (A,B). Antibodies tested include PARP-1 (N-20): sc-1561 (A) and PARP-1 (A-20): sc-1562 (B).
PARP-1 (N-20): sc-1561. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human spleen showing nuclear staining.
PARP-1 (N-20): sc-1561. Western blot analysis of PARP-1 expression in non-transfected: sc-117752 (A) and human PARP-1 transfected: sc-114869 (B) 293T whole cell lysates.
PARP-1 (N-20): sc-1561. Western blot analysis of PARP-1 expression in non-transfected: sc-117752 (A) and mouse PARP-1 transfected: sc-122382 (B) 293T whole cell lysates.
PARP-1 (N-20): sc-1561. Western blot analysis of PARP-1 expression in Jurkat (A) and HeLa (B) nuclear extracts.
