epitope mapping near the N-terminus of DEF-3 of human origin
recommended for detection of DEF-3 (also designated g16 or NY-LU-12) of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
DEF-3 Background Information DEF-3 and LUCA15 belong to an evolutionarily conserved family of RNA binding proteins and share similiar expression patterns (1). Both DEF-3 and LUCA15 are highly expressed in adult heart and thymus as well as fetal kidney (1). Conversely, fetal thymus and adult kidney express very little DEF-3 and LUCA15 (1). In the haemopoietic system of mice, the expression of DEF-3 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development (2). Both DEF-3 and LUCA15 contain two zinc finger motifs, a bipartite nuclear signal and two RNA binding motifs (3,4). DEF-3 and LUCA15 are capable of specifically binding poly(G) RNA (1). The genes encoding human DEF-3 and LUCA15 map to 3p21.3, a region homozygously deleted in the small cell lung cancer cell line GLC20 (3,5). The onset of lung cancer arises from mutations in dominant and recessive oncogenes, and chromosome 3p contains many of these recessive oncogenes (5).
