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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 448-747 mapping at the C-terminus of sirtuin 1 of human origin
- recommended for detection of SIRT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
SIRT1 |
23411 |
10q21.3 |
NM_012238 |
Q96EB6
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604479 |
| Mouse |
Sirt1 |
93759 |
10 B4 |
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Q923E4
|
N/A |
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SIRT1 Background Information
The silent information regulator (SIR2) family of genes are highly-conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 is highly expressed in heart, brain and skeletal muscle, with low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lys 382.
| SIRT1 (H-300) Product Citations |
See how others have used SIRT1 (H-300): sc-15404 antibody and or SIRT1 (H-300) antibody conjugates.
28 total citations Loading citations.
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SIRT1 (H-300)
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SIRT1 (H-300): sc-15404. Western blot analysis of SIRT1 expression in MES-SA/Dx5 whole cell lysate.
SIRT1 (H-300): sc-15404. Western blot analysis of SIRT1 expression in K-562 nuclear extract.
SIRT1 (H-300): sc-15404. Immunofluorescence staining of methanol-fixed MES-SA/Dx5 cells showing nuclear localization.
SIRT1 (H-300): sc-15404. Western blot analysis of SIRT1 expression in F9 whole cell lysate.
SIRT1 (H-300): sc-15404. Western blot analysis of SIRT1 expression in non-transfected: sc-117752 (A) and human SIRT1 transfected: sc-113797 (B) 293T whole cell lysates. SIRT1 (H-300): sc-15404. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes.
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