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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping within subdomain XI of rat ERK 2 encoded MAP kinase p42 of rat origin
- recommended for detection of ERK 2 p42 and, to a lesser extent, ERK 1 p44 of mouse, rat, human, chicken, frog, zebrafish and Drosophila origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-153 P
- phycoerythrin (sc-153 PE) conjugate for intracellular FCM, 100 tests
- Alexa Fluor® 405 (sc-153 AF405), Alexa Fluor® 488 (sc-153 AF488) and Alexa Fluor® 647 (sc-153 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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ERK 2 Background Information
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.
| ERK 2 (K-23) Product Citations |
See how others have used ERK 2 (K-23): sc-153 antibody and or ERK 2 (K-23) antibody conjugates.
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ERK 2 (K-23)
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ERK 2 (K-23): sc-153. Western blot analysis of ERK 1 and ERK 2 expression in HeLa (A), A-431 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.
ERK 2 (K-23): sc-153. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
ERK 2 (K-23) Alexa Fluor 488: sc-153 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-45068. ERK 2 (K-23) Alexa Fluor 647: sc-153 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-24647. ERK 2 (K-23): sc-153. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
ERK 2 (K-23): sc-153. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
ERK 2 (K-23): sc-153. Western blot analysis of ERK 1 expression in non-transfected: sc-117752 (A) and mouse ERK 1 transfected: sc-126806 (B) 293T whole cell lysates. ERK 2 (K-23): sc-153. Western blot analysis of ERK 2 expression in rat hippocampus tissue extract.
ERK 2 (K-23) PE: sc-153 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871. ERK 2 (K-23): sc-153. Western blot analysis of ERK 1 expression in non-transfected: sc-117752 (A) and human ERK 1 transfected: sc-171765 (B) 293T whole cell lysates. Western blot analysis of ERK 1/2 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-ERK 1/2 (Thr 177 )-R: sc-16981-R (A,B,C) and ERK 2 (K-23): sc-153 (D,E,F). Western blot analysis of ERK phosphorylation in untreated (A,C) and Sorafenib ( sc-220125) treated (B,D) SK-MEL-28 whole cell lysates. Antibodies tested include p-ERK (E-4): sc-7383 (A,B) and ERK 2 (K-23): sc-153 (C,D). Note inhibition of ERK phosphorylation by Sorafenib in lane B. Western blot analysis of ERK phosphorylation in untreated (A,C) and Sorafenib ( sc-220125) treated (B,D) SW480 whole cell lysates. Antibodies tested include p-ERK (E-4): sc-7383 (A,B) and ERK 2 (K-23): sc-153 (C,D). Note inhibition of ERK phosphorylation by Sorafenib in lane B. |
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