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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-152-G
- epitope mapping at the N-terminus of VEGF-A of human origin
- recommended for detection of the 189, 165 and 121 amino acid splice variants of VEGF of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- blocking peptide, sc-152 P
- agarose conjugate for IP studies, sc-152 AC, 500 µg/0.25 ml agarose
- also provided azide-free for biological studies, sc-152 L, 200 µg/0.1 ml
- biotin conjugate for use in Sandwich ELISA assays, sc-152 B, 200 µg/ml
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Ordering InformationProduct Citations
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VEGF Background Information
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
| VEGF (A-20) Product Citations |
See how others have used VEGF (A-20): sc-152 antibody and or VEGF (A-20) antibody conjugates.
210 total citations Loading citations.
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VEGF (A-20)
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VEGF (A-20): sc-152. Western blot analysis of reduced (A) and unreduced (B) purified VEGF protein (20 ng).
VEGF (A-20): sc-152. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human kidney showing diffuse cytoplasmic staining of kidney tubules (A). Immunofluorescence staining of PMA-treated, methanol-fixed SK-BR-3 cells, showing cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain (B).
Western blot analysis of human recombinant VEGF. Antibodies tested include VEGF (147): sc-507 (A) and VEGF (A-20): sc-152 (B). VEGF (A-20)-G: sc-152-G. Western blot analysis of human recombinant VEGF. VEGF (A-20): sc-152. Immunoperoxidase staining of formalin fixed, paraffin-embedded human soft tissue showing cytoplasmic staining of chondrocytes, endothelial cells, and perineural cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
VEGF (A-20): sc-152. Western blot analysis of VEGF expression in non-transfected: sc-117752 (A) and human VEGF transfected: sc-117031 (B) 293T whole cell lysates. VEGF(A-20): sc-152. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
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