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C/EBP β (C-19) Antibody: sc-150

 |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-150-G
  • epitope mapping at the C-terminus of C/EBP β of rat origin
  • recommended for detection of C/EBP β of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, bovine and porcine
  • blocking peptide, sc-150 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-150 X, 200 µg/0.1 ml
  • agarose conjugate for IP studies, sc-150 AC, 500 µg/0.25 ml agarose
 
Additional C/EBP Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   FCM   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CEBPB 1051 20q13.13 NM_005194 P17676
189965
Mouse Cebpb 12608 2 H3 P28033
N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
C/EBP β (C-19) sc-150 200 µg/ml $279
C/EBP β (C-19) AC sc-150 AC 500µg/ml, 25%ag $366
C/EBP β (C-19) P sc-150 P
(peptide)
100 µg/0.5 ml $61
C/EBP β (C-19) X sc-150 X 200 µg/0.1 ml $279
C/EBP β (C-19)-G sc-150-G 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
C/EBP β siRNA (h) sc-29229 10 µM $258
C/EBP β siRNA (m) sc-29862 10 µM $258
C/EBP β siRNA (h2) sc-44251 10 µM $258
C/EBP β (h)-PR sc-29229-PR 10 µM $23
C/EBP β (m)-PR sc-29862-PR 10 µM $23
C/EBP β (h2)-PR sc-44251-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
C/EBP β shRNA Plasmid (h) sc-29229-SH 20 µg $520
C/EBP β shRNA Plasmid (m) sc-29862-SH 20 µg $520
C/EBP β shRNA Plasmid (h2) sc-44251-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
C/EBP β shRNA (h) Lentiviral Particles sc-29229-V 200 µl $625
C/EBP β shRNA (m) Lentiviral Particles sc-29862-V 200 µl $625
C/EBP β shRNA (h2) Lentiviral Particles sc-44251-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
Jurkat nuclear extract sc-2132 1000 µg $143
HeLa nuclear extract sc-2120 1000 µg $143
mouse liver extract sc-2256 500 µg/200 µl $104
Hep G2 Cell Lysate sc-2227 500 µg/200 µl $104

C/EBP β Background Information
CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP ∫ is a member of the C/EBP transcription factor family. The C/EBP ∫ gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. C/EBP ∫ is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP ∫ contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP ∫ gene that has direct implication for acute phase response in liver cells.The C/EBP ∫ form requires phosphorylation for its DNA binding ability, and increase binding of C/EBP ∫ isoforms during acute-phase reaction occurs through its upregulation and structural modification.

C/EBP β (C-19) Product Citations
See how others have used C/EBP β (C-19): sc-150 antibody and or C/EBP β (C-19) antibody conjugates.


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C/EBP β (C-19)
Click on image to enlarge
C/EBP β (C-19): sc-150. Western blot analysis of C/EBP β expression in control (A) and phorbol-induced (B) Jurkat cell nuclear extracts. C/EBP β fusion protein is shown as positive control (C).
C/EBP β (C-19): sc-150. Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.
C/EBP β (C-19): sc-150. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with C/EBP β (C-19), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
C/EBP β (C-19): sc-150. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with C/EBP β (C-19), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
C/EBP β (C-19): sc-150. Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
C/EBP β (C-19): sc-150. Western blot analysis of C/EBP β expression in non-transfected 293T: sc-117752 (A), human C/EBP β transfected 293T: sc-176940 (B) and Jurkat (C) whole cell lysates.
C/EBP β (C-19): sc-150. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with C/EBP β (C-19), followed by PE-conjugated chicken anti-rabbit IgG: sc-3762. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
C/EBP β (C-19): sc-150. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with C/EBP β (C-19), followed by FITC-conjugated bovine anti-rabbit IgG: sc-2365. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
C/EBP β (C-19): sc-150. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with C/EBP β (C-19),followed by PerCP-conjugated goat anti-rabbit IgG: sc-45090. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
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