epitope mapping within an internal region of NEDD4-1 of human origin
recommended for detection of NEDD4-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
NEDD4-1 Background Information NEDD4 family interacting protein-1 (NDFIP1), also referred to as N4WBP5, is a member of a family of highly conserved proteins. It has three transmembrane domains in its carboxy-terminal and two PY motifs in its amino-terminal. NDFIP1 is a 221 amino acid, Golgi-associated protein that may play a role in Golgi structure and function. NDFIP1 binds the WW domains of a number of NEDD4 family members called HECT-type E3 ubiquitin ligases. NDFIP1 is strongly expressed in surviving neurons around a site of injury. This suggests that ubiquitination may be a possible survival strategy and NDFIP1 may act as a neuroprotective protein. NDFIP1 may also have an effect on the function of Itch, another E3 ubiquitin ligase. Expression of NDFIP1 and its association with Itch may be promoted by T cell activation. Mice lacking NDFIP1 exhibit inactivation of Itch and accumulation of Jun B causing them to die prematurely with severe skin and lung inflammation.
