epitope mapping within an internal region of REV1 of human origin
recommended for detection of REV1 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
blocking peptide, sc-13827 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-13827 X, 200 µg/0.1 ml
REV1 Background Information Originally identified in Saccaromyces cerevisiae, Rev1p exhibits deoxycytidyl transferase activity and is required for translesion replication and mutagenesis induced by a wide variety of DNA-damaging events (1-3). The human homolog REV1, like its yeast Rev1p counterpart, is also involved in translesion replication and spontaneous mutagenesis (1,4,5). The human REV1 gene maps between the chromosomal loci 2q11.1 and 2q11.2 and is ubiquitously expressed in various human tissues (6). Human REV1 protein is a dCMP transferase that specifically inserts a dCMP residue either opposite a DNA template guanine, a DNA template apurinic/apyridinic site or a uracil residue (6). REV1 transferase may play a critical role during mutagenic translesion DNA synthesis by bypassing a template adenosine/guanine site in human cells (6).
