directed to the 26 kDa GST specific domain of a fusion protein encoded by a pGEX.3X recombinant vector
recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins
WB and IP of recombinant GST fusion proteins expressed in E. coli; specifically designed to be used in combination with GST expression vectors such as pGEX.3X and pGEX.2T (Smith and Johnson, Gene 67: 31, 1988)
agarose conjugate for IP studies, sc-138 AC, 500 µg/0.25 ml agarose
fluorescein (sc-138 FITC) and rhodamine (sc-138 TRITC) conjugates for immunofluorescence, 200 µg/ml
HRP conjugate, sc-138 HRP, 200 µg/ml
biotin conjugate, sc-138 B, 200 µg/ml
Alexa Fluor® 405 (sc-138 AF405), Alexa Fluor® 488 (sc-138 AF488) and Alexa Fluor® 647 (sc-138 AF647) conjugates for immunofluorescence; 100 µg/2 ml
GST Background Information Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
GST (B-14) Product Citations
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GST (B-14)
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GST (B-14): sc-138. Western blot analysis of GST-tagged fusion proteins showing C-terminal GST-tagged Max (A) and N-terminal GST-tagged Bcl-6 (B).
GST (B-14): sc-138. Western blot analysis of human recombinant NFκB p50 fusion protein.
