GAPDH Antibody (A-3): sc-137179
- GAPDH Antibody (A-3) is a mouse monoclonal IgG1 κ, cited in 212 publications, provided at 200 µg/ml
- raised against amino acids representing full length GAPDH of human origin
- Anti-GAPDH Antibody (A-3) is recommended for detection of GAPDH and GAPDH-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- GAPDH Antibody (A-3) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GAPDH (A-3): sc-137179.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GAPDH Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with GAPDH Antibody (A-3) (see ordering information below).
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GAPDH Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody raised against full-length human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), recognizing GAPDH and GAPDH-2 in mouse, rat, and human samples. Anti-GAPDH antibody (A-3) is suitable for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GAPDH is a key glycolytic enzyme catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate, an essential step in carbohydrate metabolism. Beyond glycolytic function, GAPDH undergoes various post-translational modifications (PTMs) such as S-nitrosylation, acetylation, phosphorylation, ubiquitination, and ADP-ribosylation, which modulate activity, localization, and protein-protein interactions. These modifications are crucial for enabling GAPDH′s roles in diverse cellular processes, including transcription regulation, DNA repair, and nuclear RNA export. For example, S-nitrosylation facilitates GAPDH′s translocation to the nucleus during apoptosis, linking GAPDH to programmed cell death pathways. Similarly, acetylation and phosphorylation can regulate GAPDH′s involvement in cellular stress responses and protein aggregation, particularly in neurodegenerative diseases like Alzheimer′s and Huntington′s. PTMs also affect GAPDH expression and function in cancer, where GAPDH is often upregulated and contributes to tumor progression. GAPDH (A-3) antibody is an indispensable tool for investigating how PTMs influence GAPDH′s multifaceted roles in health and disease, advancing research in metabolism, cancer, and neurodegeneration.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GAPDH Antibody (A-3) References:
- Over-expression of GAPDH induces apoptosis in COS-7 cells transfected with cloned GAPDH cDNAs. | Tajima, H., et al. 1999. Neuroreport. 10: 2029-33. PMID: 10424669
- Glyceraldehyde-3-phosphate dehydrogenase and apoptosis. | Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
- Maternal diabetes in vivo and high glucose in vitro diminish GAPDH activity in rat embryos. | Wentzel, P., et al. 2003. Diabetes. 52: 1222-8. PMID: 12716756
- Exportin 1-independent nuclear export of GAPDH. | Schmitz, HD., et al. 2003. Cell Biol Int. 27: 511-7. PMID: 12842090
- Experimental immunological infertility effect of anti-GAPDH-2 antibodies on the fertility of female mice. | Wang, Y., et al. 2009. Fertil Steril. 92: 2020-7. PMID: 18980760
- The multifunctional glycolytic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a novel macrophage lactoferrin receptor. | Rawat, P., et al. 2012. Biochem Cell Biol. 90: 329-38. PMID: 22292499
- CIB1 prevents nuclear GAPDH accumulation and non-apoptotic tumor cell death via AKT and ERK signaling. | Leisner, TM., et al. 2013. Oncogene. 32: 4017-27. PMID: 22964641
- S-nitrosylation of B23/nucleophosmin by GAPDH protects cells from the SIAH1-GAPDH death cascade. | Lee, SB., et al. 2012. J Cell Biol. 199: 65-76. PMID: 23027902
- Enhancement by GOSPEL protein of GAPDH aggregation induced by nitric oxide donor and its inhibition by NAD(.). | González, MC., et al. 2016. FEBS Lett. 590: 2210-20. PMID: 27282776
- Reduction of GAPDH in lenses of Parkinson's disease patients: A possible new biomarker. | Klettner, A., et al. 2017. Mov Disord. 32: 459-462. PMID: 27859598
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAPDH Antibody (A-3) | sc-137179 | 200 µg/ml | $316.00 | |||
GAPDH Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-527127 | 200 µg Ab; 10 µg BP | $354.00 | |||
GAPDH Antibody (A-3): m-IgG1 BP-HRP Bundle | sc-532500 | 200 µg Ab; 20 µg BP | $354.00 | |||
GAPDH Antibody (A-3) AC | sc-137179 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GAPDH Antibody (A-3) FITC | sc-137179 FITC | 200 µg/ml | $330.00 | |||
GAPDH Antibody (A-3) PE | sc-137179 PE | 200 µg/ml | $343.00 | |||
GAPDH Antibody (A-3) Alexa Fluor® 488 | sc-137179 AF488 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (A-3) Alexa Fluor® 546 | sc-137179 AF546 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (A-3) Alexa Fluor® 594 | sc-137179 AF594 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (A-3) Alexa Fluor® 647 | sc-137179 AF647 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (A-3) Alexa Fluor® 680 | sc-137179 AF680 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (A-3) Alexa Fluor® 790 | sc-137179 AF790 | 200 µg/ml | $357.00 |