CstF-50 (D-15) Antibody: sc-13658

CstF-50 Background Information
Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors (1,2). The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits (2,3). Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA (4). The shortest CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44-amino acid-long sequence that is repeated seven times (5). CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro (1). CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3’-end cleavage factors to form a stable complex on the pre-mRNA (5).