Welcome to Santa Cruz Biotechnology!
SCBT Home
Home » Kinases and Phosphatases » MEK Antibodies » p-MEK-1 Antibody (47.Ser 222): sc-136542

p-MEK-1 Antibody (47.Ser 222): sc-136542

 |  Datasheet

(Based on data analysis)

  • p-MEK-1 Antibody (47.Ser 222) is a mouse monoclonal IgA provided at 200 µg/ml
  • raised against a short amino acid sequence containing phosphorylated Ser 222 of MEK-1 of human origin
  • recommended for detection of Ser 222 phosphorylated MEK-1 of human origin and Ser 222 phosphorylated MEK-2 of human origin by WB, IP and ELISA
 

See additional MEK Antibodies including MEK, MEK, MEK-1, MEK-2, MEK-3, MEK-4, MEK-5, MEK-6 and MEK-7.

Also see MEK-1 Inhibitors for functional analysis of cellular responses to MEK-1.


Ordering InformationGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-MEK-1 Antibody (47.Ser 222) sc-136542 200 µg/ml $279
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAP2K1 5604 15q22.31 NM_002755 NP_002746
 176872
 

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38ß (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAFor MEKK1) positively regulates the kinase activity.
p-MEK-1 Antibody (47.Ser 222) Data
Click on image to enlarge
sc-136542
Western blot analysis of MEK-1 phosphorylation in untreated (A,D), serum-starved, PMA-treated (B,E), serum-starved, PMA and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1 (47.Ser 222): sc-136542 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).
Download
Datasheets
Get Adobe Acrobat Reader