p-Cdc2 p34 Antibody (pY15.44): sc-136014

p-Cdc2 p34 Antibody (pY15.44) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-Cdc2 p34 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-p-Cdc2 p34 antibody (pY15.44) is available as the non-conjugated format. p-Cdc2 p34 protein, also known as p-CDK1, plays a crucial role in regulating the cell cycle, particularly in the transition from G2 phase to M phase. p-Cdc2 p34 is a serine/threonine-specific protein kinase that is tightly regulated through interaction with B-type cyclins and reversible phosphorylation. Binding of Cyclin B to Cdc2 p34 is essential for activation, promoting phosphorylation of Cdc2 at three key regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, phosphorylation of threonine 14 and tyrosine 15 serves as a negative regulatory mechanism, preventing premature entry into mitosis. Conversely, phosphorylation of threonine 161 by cyclin-dependent kinases is necessary for Cdc2 activation and progression through the cell cycle. Precise regulation of these phosphorylation events is vital for ensuring proper cell division and maintaining genomic stability, highlighting p-Cdc2 p34′s importance in cellular function and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Cdc2 p34 Antibody (pY15.44) References:

1. Vertebrate p34cdc2 phosphorylation site mutants: effects upon cell cycle progression in the fission yeast Schizosaccharomyces pombe.  |  Krek, W., et al. 1992. J Cell Sci. 102 (Pt 1): 43-53. PMID: 1500441
2. Gambogic acid-induced G2/M phase cell-cycle arrest via disturbing CDK7-mediated phosphorylation of CDC2/p34 in human gastric carcinoma BGC-823 cells.  |  Yu, J., et al. 2007. Carcinogenesis. 28: 632-8. PMID: 17012222
3. Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites.  |  Krek, W. and Nigg, EA. 1991. EMBO J. 10: 305-16. PMID: 1846803
4. Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate.  |  Draetta, G., et al. Nature. 336: 738-44. PMID: 2462672
5. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
6. cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.  |  Arion, D., et al. 1988. Cell. 55: 371-8. PMID: 2844417
7. Identification of p34 and p13, human homologs of the cell cycle regulators of fission yeast encoded by cdc2+ and suc1+.  |  Draetta, G., et al. 1987. Cell. 50: 319-25. PMID: 3297353
8. p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.  |  Brizuela, L., et al. 1987. EMBO J. 6: 3507-14. PMID: 3322810
9. Mechanisms of p34cdc2 regulation.  |  Atherton-Fessler, S., et al. 1993. Mol Cell Biol. 13: 1675-85. PMID: 8441405
10. Crystal structure of cyclin-dependent kinase 2.  |  De Bondt, HL., et al. 1993. Nature. 363: 595-602. PMID: 8510751

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Cdc2 p34 Antibody (pY15.44) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Cdc2 p34 Antibody (pY15.44) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Cdc2 p34 Antibody (pY15.44) Specifications

Alternate Product Name: Cdc2 p34

Product Clone Name: pY15.44

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 15

Epitope Target: Cdc2 p34

Epitope Species: human

Product Reactivity/Detection: Tyr 15 phosphorylated Cdc2 p34

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for p-Cdc2 p34 Antibody (pY15.44): N/A

The preferred Cdc2 p34 antibody is Cdc2 p34 Antibody (17): sc-54.