p-Pol II (8A7) Antibody sc-13583

santa cruz biotechnology, inc.

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p-Pol II (8A7) Antibody: sc-13583	\| Datasheet

mouse monoclonal IgM, 200 µg/ml
raised against synthetic peptide: 10 repeats of YSPTSpPS (Human)
recommended for detection of Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP and IF
TransCruz reagent for Gel Supershift and ChIP applications, sc-13583 X, 200 µg/0.1 ml

Additional RNA Polymerase Antibodies ...

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Recommended Support Products:

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WB IP IF Gel Shift ChIP

Species	Gene Name	Gene ID	Chromosome Location	Protein Accession #	OMIM™ Number

Mouse	Polr2a	20020	11 B3	P08775	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price
p-Pol II (8A7)	sc-13583	200 µg/ml	$279
p-Pol II (8A7)	sc-13583 X	200 µg/0.1 ml	$279
Lambda Phosphatase	sc-200312	100,000 units	$462
Lambda Phosphatase	sc-200312A	20,000 units	$116

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
HeLa + Calyculin A Cell Lysate	sc-2271	500 µg/200 µl	$104
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104
HeLa + heat shock Cell Lysate	sc-2272	500 µg/200 µl	$104

Pol II Background Information
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

p-Pol II (8A7) Product Citations

See how others have used p-Pol II (8A7): sc-13583 antibody and or p-Pol II (8A7) antibody conjugates.

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p-Pol II (8A7)

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p-Pol II (8A7): sc-13583. Western blot analysis of Pol II phosphorylation in untreated (A), heat shocked (C) and calyculin-treated (E) HeLa cultures. Total Pol II levels were determined in the same respective extracts by using Pol II (A-10): sc-17798 (B,D,F).

p-Pol II (8A7): sc-13583. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p-Pol II (8A7): sc-13583. Western blot analysis of Pol II phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HeLa whole cell lysates.

Western blot analysis of Pol II phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Pol II (8A7): sc-13583 (A,B,C) and Pol II (8WG16): sc-56767 (D,E,F).

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p-Pol II (8A7) Antibody: sc-13583