AF-6 Background Information The dynamics of a cell-cell interface such as tight junctions or adherens junctions are important in many developmental, physiological, and pathological processes. AF-6 (MLLT4: myeloid/lymphoid or mixed-lineage leukemia trans-located to 4) is a 1,612 amino acid protein that contains two N-terminal Ras binding domains (RBD) and a GLGF motif, and is implicated in Ras-mediated signaling events occurring at peripheral cell-cell junctions. AF-6 interacts with F-actin and Profilin in cell-cell junctions, and may modulate Actin modeling near adhesion complexes. Furthermore, AF-6 coordinates junction adhesion molecule (JAM) recruitment to intercellular junctions through a PDZ domain. Developing mice deficient in AF-6 activity display a loss of neuroepithelial polarity, suggesting that AF-6 activity is an important regulator of cell-cell junctions that influence apical/basolateral asymmetry.
AF-6 (35)
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AF-6 (35): sc-135823. Western blot analysis of AF-6 expression in PC-12 whole cell lysate.
