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p-Raf-1 Antibody (Ser 301): sc-135707

 |  Datasheet

(Based on data analysis)

  • p-Raf-1 Antibody (Ser 301) is a rabbit polyclonal IgG provided at 100µl
  • recommended for detection of Ser 301 phosphorylated Raf-1 of mouse, rat, human and Xenopus origin by WB and IP; also reactive with additional species, including bovine and canine
 

See additional Raf Antibodies including Raf, A-Raf, Raf-1 and Raf-B.

Also see Raf-1 Inhibitors, Raf-1 Activators or Raf-1 Substrates for functional analysis of cellular responses to Raf-1.


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WB   IP   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-Raf-1 Antibody (Ser 301) sc-135707 100 µl $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Raf-1 siRNA (h) sc-29462 10 µM $258
Raf-1 siRNA (m) sc-29463 10 µM $258
Raf-1 (h)-PR sc-29462-PR 10 µM, 20 µl $23
Raf-1 (m)-PR sc-29463-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Raf-1 shRNA Plasmid (h) sc-29462-SH 20 µg $520
Raf-1 shRNA Plasmid (m) sc-29463-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Raf-1 shRNA (h) Lentiviral Particles sc-29462-V 200 µl $625
Raf-1 shRNA (m) Lentiviral Particles sc-29463-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Raf-1 (h2): 293T Lysate sc-113164 100 µg/200 µl $205
Raf-1 (m): 293T Lysate sc-122942 100 µg/200 µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
CCD-1064Sk Cell Lysate sc-2263 500 µg/200 µl $104
CCD-1064Sk + PDGF Cell Lysate sc-2264 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
A-431 + EGF Whole Cell Lysate sc-2202 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human RAF1 5894 3p25.1 P04049
 611554
Mouse Raf1 110157 6 E3 Q99N57
 N/A
 

Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338. Mouse, rat and human Raf-1 are phosphorylated, upon DNA damage, on Ser 301.

p-Raf-1 Antibody (Ser 301) Data
Click on image to enlarge
sc-135707
p-Raf-1 (Ser 301): sc-135707. Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) UV-treated Jurkat whole cell lysates.
sc-135707
p-Raf-1 (Ser 301): sc-135707. Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) NIH/3T3 whole cell lysates.
sc-135707
p-Raf-1 (Ser 301): sc-135707. Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-110760 (A) and human Raf-1 transfected: sc-113164 (B) 293 whole cell lysates.
sc-135707
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Raf-1 transfected: sc-122942 (B, E) and lambda protein phosphatase treated mouse Raf-1 transfected: sc-122942 (C, F) 293T whole cell lysates. Antibodies tested include p-Raf-1 (Ser 301): sc-135707 (A, B, C) and Raf-1 (540): sc-52827 (D, E, F).
sc-135707
Western blot analysis of Raf-1 phosphorylation in non-transfected: sc-110760 (A,D), untreated human Raf-1 transfected: sc-158911 (B,E) and lambda protein phosphatase (sc-200312A) treated human Raf-1 transfected: sc-158911 (C,F) 293 whole cell lysates. Antibodies tested include p-Raf-1 (Ser 301): sc-135707 (A,B,C) and Raf-1 (540): sc-52827 (D,E,F).
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